From: The association of complex liver disorders with HBV genotypes prevalent in Pakistan
 | Primers | Sequence | Gene/CDS | Product |
---|---|---|---|---|
Step-one PCR | P1b universal, sense) | 5'-TCA CCA TAT TCT TGG GAA CAA GA-3' | nt2823-2845, | 1065 bp |
 | S1-2 universal, antisense) | 5'-CGA ACC ACT GAA CAA ATG GC-3' | nt685-704, |  |
Step-two PCR | B2 sense | 5'-GGC TCM AGT TCM GGA ACA GT-3' | nt67-86, types A specific, to E | Â |
Mix A | BAIR antisense | 5'-CTC GCG GAG ATT GAC GAG ATG T-3' | nt113-134, type A specific, | 68 bp |
 | BBIR antisense | 5'-CAG GTT GGT GAG TGA CTG GAG A-3' | nt324-345, type B specific, | 122 bp |
 | BCIR antisense) | 5'-GGT CCT AGG AAT CCT GAT GTT G-3' | nt165-186, type C specific, | 281 bp |