Figure 5From: The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopmentThe effect of UL20p carboxyl terminal truncations on UL20p and gK colocalization in TGN cellular compartments. As with figure 4, Vero cells were co-transfected with gKD1V5, as well as with plasmids encoding wild-type or mutant UL20DIFLAG proteins. Thirty-six hours post-transfection, cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, antibodies a3xFLAG, aV5 and aTGN46 were used to identify, UL20p, gK and TGN46, respectively.Back to article page