Figure 4From: The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopmentThe effect of UL20p amino terminal mutations on UL20p and gK colocalization in TGN cellular compartments. Vero cells were co-transfected with gK tagged with the V5 epitope (D1V5), as well as with plasmids encoding wild-type or different mutant UL20ps tagged with the 3 × FLAG epitope (UL20D1FLAG). Thirty-six hours post-transfection, cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, rabbit anti-FLAG (α FLAG) mAb was used to detect UL20p, mouse anti-V5 (α V5) epitope was used to detect gK, and sheep aTGN46 mAb was used to identify the TGN. First three rows of the confocal pictures show co-localization of UL20p with gK, while rows 4–6 show colocalization of gK with TGN46.Back to article page