Figure 5

Histological changes in livers of mice infected with 'scid-adapted' MARV. Scid mice were challenged IP with 1000 pfu of 'scid-adapted' MARVCi67 and tissue samples were collected on days 0, 2, 4, 6, and 8 after challenge (n = 4–5/group). (A, C, and E) Tissues from the MARV-infected mice were stained with hematoxylin and eosin and representative pictures from day 0 (A), 4 (C), and 6 (E) are shown. The liver from the MARV-infected mouse contains multifocal necrosis, hepatocellular disruption, fatty cell degeneration, scattered hepatocellular viral inclusions, and inflammation composed of variable numbers of neutrophils and lesser numbers of macrophages and lymphocytes. (B, D, and F) Immunohistochemistry was performed on tissue sections from days 0 (B), 4 (D), and 6 (F) and MARV antigen appears brown. In the liver, MARV antigen is localized at the hepatocellular surface and most prominently noted along the sinusoids. Magnifications for A-B and D-F were 20× and panel C is shown at 40×.