Figure 3From: Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoproteinRole of the coiled coil region for interaction of NP with VP35. (A) Mutants of NP (Fig. 1) were in vitro translated and metabolically labeled with [35S]ProMix. Samples were separated by SDS-PAGE and analyzed using a BioImager. (B) In vitro translated and [35S]ProMix metabolically labeled mutants of NP (Fig. 1A) were incubated with bacterially expressed GST-VP35 or GST (negative control). Complexes were pulled down with glutathion-sepharose, separated on SDS-PAGE and analyzed using a BioImager. Binding of NP to GST-VP35 was set to 100%. (C) Quantification of 3 separate experiments as shown under (B).Back to article page