Figure 3
PTD tat modification promoted Ad5 binding to cell surfaces. Binding of Ad5 and Ad5.PTDtat were examined in both high-CAR expressing Hela cells (A) and low-CAR expressing U118MG cells (B) at 4°C. The amount of viruses associated with the cells was determined by quantitative PCR after DNA isolation from the cell lysate, and the viral copy numbers were normalized to actin DNA in the samples. The * indicates p < 0.05 and ** indicates p < 0.01 as analyzed by the Student's t-test.