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Table 2 Plasimid Rescued influenza A & B Viruses In MDCK cellsa

From: Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells

Type

Strain

Virus titer (pfu/ml)

A

  

   H1N1

ca A/New Caledonia/20/1999

7. 0 × 106

 

ca A/Solomon Island/3/2006

5.4 × 107

   H2N2

MDV-A (ca A/Ann Arbor/6/1960)

1.5 × 108

   H3N2

ca A/Wisconsin/67/2005

8.4 × 106

 

ca A/California/7/2004

2.6 × 106

 

ca A/Panama/2007/1999

9. 0 × 106

   H5N1

ca A/Hong Kong/213/2003

1.3 × 107

 

ca A/Hong Kong/1997(491 H5/486 N1)

1.7 × 107

 

ca A/Vietnam/1203/2004

4.6 × 107

   H6N1

ca A/Teal/HK/W312/1997

6.0 × 106

   H7N3

ca A/CK/BC-CN/2004

3.9 × 106

   H9N2

ca A/chicken/HK/G9/1997

1.4 × 107

B

  
 

MDV-B (B/Ann Arbor/1/1966)

6.0 × 107

 

MDV-B (B/Ann Arbor/1/1966)-Mutant

1.5 × 107

   Victoria

ca B/Hong Kong/330/2001

1.7 × 107

 

ca B/Malaysia/2506/2004

1.6 × 107

   Yamagata

ca B/Jiangsu/10/2003

5.3 × 107

 

ca B/Florida/07/2004

2.6 × 107

  1. a Supernatants of transfected MDCK cells were collected on days 5, 6, and 7 after transfection. Virus titer was determined by plaque assay for the supernatant collected on the day post-transfection when the MDCK cells exhibited 50 to 100% CPE.
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Virology Journal

ISSN: 1743-422X