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Figure 7 | Virology Journal

Figure 7

From: Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells

Figure 7

Map of pAD4000. The MDCK RNA pol I/CMV pol II bidirectional vector, pAD4000, was derived from pAD3000 [3], the human pol I/CMV pol II bidirectional vector, by replacement of the human pol I promoter sequence in pAD3000 with the MDCK 469 bp sequence upstream of the transcription initiation site. In addition, the two BsmBI restriction sites in pAD3000, which are used for cloning sequences between the two promoters, were changed to BbsI sites because the former restriction site occurs in the MDCK pol I containing fragment.

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Virology Journal

ISSN: 1743-422X