Figure 5From: Quantitative analysis of Nipah virus proteins released as virus-like particles reveals central role for the matrix proteinImmunoelectron microscopy shows particles consistent in size with authentic NiV virions. VLPs released from transfected 293T cells were purified by gradient centrifugation, labeled by immuno-gold, negatively stained, and viewed by electron microscopy as described in Methods. VLPs were derived from cells that expressed M (A to C) or N, M, F, and G (D and E). Immunolabeling against M was done using MAb F45G5 (A to D) and G was detected with mouse anti-HeV G polyclonal serum (E). Bars represent 100 nm. Specificity of primary and secondary antibodies was demonstrated by their failure to stain negative controls (not shown).Back to article page