Figure 3

Activation-induced EGFP expression in Jurkat cells transduced with SINIL-2pr retroparticles. A-D. Flow cytometry analysis of mean EGFP expression in Jurkat T-cells transduced with SINIL-2pr retroparticles relative to Jurkat null cells. Expression was measured at ~48 hr post-activation with 1 μM ionomycin and 10 ng/ml PMA relative to untreated cells. E. Cyclosporin A-sensitive induction of EGFP expression in Jurkat cells transduced with SINIL-2pr. Co-stimulation of the SINIL-2pr-gene modified Jurkat T-cells with 1 μM ionomycin and 10 ng/ml PMA resulted in a 2.0 ± 0.1 – fold increase in relative mean EGFP expression measured at ~48 hrs (P = 0.011). This activation-induced EGFP expression was abrogated when the cells were pretreated for ~30 minutes with 300 nM CsA.