Table 2 Primer sequences used for the amplification of MV and housekeeping genes with amplicon length.
From: Measles viral load may reflect SSPE disease progression
Gene (GenBank accession number) | Primer sequence 5'-3' | Expected amplicon length |
|---|---|---|
MV N | CAA GAC CCT GAG GGA TTC AA GTT CCT CAC CAC ATC CAA CC | 192 bp |
MV M | TGT TAC CCG CTG ATG GGT AT TTC TGG CTG TCA TTG TGA GG (M3'-1R, Jin 2002) | 223 bp |
MV H | ATC AGG GAT CAG GGA AAG GT CAT TCG CAA CTT GTC ATC TG (Mh4, Jin 1998) | 206 bp |
GAPDH (NM_002046) | GAG TCA ACG GAT TTG GTC GT TTG ATT TTG GAG GGA TCT CG | 238 bp |
beta-actin (NM_001101) | GGA CTT CGA GCA AGA GAT GG AGC ACT GTG TTG GGC GTA CAG | 234 bp |
18S rRNA (X03205) | CAT GGC CGT TCT TAG TTG GT CGC TGA GCC AGT CAG TGT AG | 218 bp |