Table 1 Reproducibility of the SYBR® Green I dye-based qRT-PCR assay for the detection and quantification of Nipah virus RNA. Intra- and inter-assay variations were calculated using duplicates and at least 14 replicates, respectively.
From: Quantitative estimation of Nipah virus replication kinetics in vitro
CV (%) | PFU/mL | |||||||
|---|---|---|---|---|---|---|---|---|
1 × 10-1 | 1 × 100 | 1 × 101 | 1 × 102 | 1 × 103 | 1 × 104 | 1 × 105 | 1 × 106 | |
Intra-assay | 3.27 | 1.20 | 1.67 | 0.06 | 0.43 | 0.01 | 0.79 | 0.96 |
Inter-assay | 4.76 | 3.55 | 2.72 | 3.52 | 1.26 | 1.27 | 2.11 | 2.86 |