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Table 1 Effect of IFN-α and IFN-γ on HSV-2 and HSV-1 plaque formation on Vero cells.

From: The synergistic effect of IFN-α and IFN-γ against HSV-2 replication in Vero cells is not interfered by the plant antiviral 1-cinnamoyl-3, 11-dihydroxymeliacarpin

 

Treatment (IU/ml)a

Mean no. of plaquesb ± SEM

Fold reductionc

HSV-2 MS

Vehicle

137 ± 8.3

-

 

IFN-α (100)

82.5 ± 6.4

1.7

 

IFN-γ (100)

86 ± 6.6

1.6

 

IFN-α (100)+IFN-γ (100)

36.5 ± 4.3

3.8

 

IFN-α (200)

68 ± 5.8

2

 

IFN-γ (200)

72 ± 6

1.9

HSV-2 G

Vehicle

98.5 ± 7

-

 

IFN-α (100)

81 ± 6.4

1.2

 

IFN-γ (100)

98 ± 7

1

 

IFN-α (100) + IFN-γ (100)

11.5 ± 2.4

8.6

 

IFN-α (200)

52 ± 5.1

1.9

 

IFN-γ (200)

35 ± 4.2

2.8

HSV-1 KOS

Vehicle

133 ± 8.2

-

 

IFN-α (100)

68.5 ± 5.9

1.9

 

IFN-γ (100)

74.5 ± 6.1

1.8

 

IFN-α (100) + IFN-γ (100)

4 ± 1.4

33

 

IFN-α (200)

54.5 ± 5.2

2.4

 

IFN-γ (200)

84.5 ± 6.5

1.6

HSV-1 F

Vehicle

100 ± 7.1

-

 

IFN-α (100)

82.5 ± 6.4

1.2

 

IFN-γ (100)

84.5 ± 6.5

1.2

 

IFN-α (100) + IFN-γ (100)

19 ± 3.1

5.3

 

IFN-α (200)

51.5 ± 5.1

1.9

 

IFN-γ (200)

53 ± 5.1

1.9

  1. aVero cells were treated continuously with IFN-α, IFN-γ or combinations of these cytokines from 16 h before infection until the end of the experiment.
  2. b Average number of plaques per well of Vero cells inoculated with 200 PFU of HSV-1 KOS and F and HSV-2 MS and G. Values represent (mean ± SEM) from three independent experiments.
  3. c Fold-reduction in each group was calculated as follows: number of plaques in vehicle/number of plaques in treatments. Values represent mean from three independent experiments. P < 0.05, as determined by one-way ANOVA and Turkey's post hoc t test comparison of this treatment to vehicle.
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Virology Journal

ISSN: 1743-422X