Figure 1

Amino acid alignment of conserved regions of the DNA polymerase of selected viruses and representative primers designed for this study. Upper primers are displayed 5'-3' and the lower primer is displayed 3'-5'. Underlined lower case nt represent 5' regions with no homology to coding region. The ~ 1200 bp and 400–700 bp following the adeno and HV primers indicate the respective distance to the region with homology to the lower primer. Represented sequences are: Aviadenovirus – fowl adenovirus A [GenBank:NP_043878], Mastadenovirus-human adenovirus C [GenBank:NP_040516], Atadenovirus-duck adenovirus 1 [GenBank:NP_044702], Siadenovirus-frog adenovirus [GenBank:NP_062435], α Herpesvirus-human herpesvirus 1 [GenBank:NP_044632], β Herpesvirus1-human herpesvirus 5 [GenBank:P08546], β Herpesvirus2-human herpesvirus 6 [GenBank:NP 042931], γ Herpesvirus-human herpesvirus 4 [GenBank:NP_039908], Ictalurid HV-Ictalurid herpesvirus 1 [GenBank:NP_041148], Ranid HV-ranid herpesvirus 1 [GenBank:AAD12269], Ostreid HV-Ostreid herpesvirus 1 [GenBank:AAS00986], African SFV-African swine fever virus [GenBank:NP_042783], Avipoxvirus-fowlpox virus [GenBank:NP_039057], Orthopoxvirus-Vaccinia [GenBank:NP_063712], Entomopoxvirus-Melanoplus sanguinipes entomopoxvirus [GenBank:NP_048107], Lymphocystivirus-lymphocystis disease virus 1 [GenBank:NP_078724], Ranavirus-frog virus 3 [GenBank:YP_031639], Iridovirus-Invertebrate iridescent virus 6 [GenBank:NP_149500], Chloriridovirus-Invertebrate iridescent virus 3 [GenBank:CAC84133], Ascovirus-Heliotis virescens ascovirus [GenBank:AJ312696]. Granulovirus1-Cryptophlebia leucotreta granulovirus [GenBank:NP_891948], Granulovirus2-Xestia c-nigrum granulovirus [GenBank:AAF05246], Nucleopolyhedrovirus1-Lymantria dispar nucleopolyhedrovirus [GenBank:NP_047720], Nucleopolyhedrovirus2-Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus [GenBank:Q83948], Whispovirus-shrimp white spot syndrome virus [GenBank:AAK77696].