Skip to main content

Table 1 Mutagenesis primers used to examine the role of the PMV replicase motif in virus accumulation.

From: Panicovirus accumulation is governed by two membrane-associated proteins with a newly identified conserved motif that contributes to pathogenicity

Mutant

Primer

Sequence (5'-3')a

F313A

REP/F1-A

CCCCAGCGGC TTCGTTCTTTGC

F1313F2

F1-313-F2

GGAACCCCAGCA AACTCGTTCTTTGC

P317A

P317A-989R

CTGTGGGTTTTGC AACCCCAGCG

N323A

N323A-1007R

CAGCCAACTGGGC AGCCTCTGTG

L325A

L325A-1014R

CTCCAGACAGCCGC CTGGTTAGC

Y330A

REP/Y-A

CACACCCTGTAGAGGGC TCTCCAG

C335A

1044R-C/A

CCTTTCTTATCAGC CACCCTGTAGAG

F357A

REP/F-A

GGAATACAGC TGGCAAGGC

D363A

REP/D-A

TGATCCTGGG CGTATGCGC

P399A

MUTPMV-1236R

GCCCCAACTAATGC ATTGGTCACTAG

W405A

REP/W-A

CCAAGCAGTCGC ATTGGCCCC

  1. a. The altered nucleotides on the PMV cDNA are underlined.
Back to article page

Virology Journal

ISSN: 1743-422X