Figure 2From: Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding proteinPositive Yeast Colonies with Potential HBX Interacting Proteins. Comparison between control yeast colonies and positive colonies from the library screening that developed blue colour over a 5-day incubation period at 30°C. A1-5, B1-5, C1-5, D1-5, E1-5, F1, G6-10, H6-10, I6-10, J6-10, K6-10, L6: colonies that turn blue after α-galactosidase assay screening indicated positive interaction between bait and cDNA library. F2 and L7: control using bait plasmid cloned with HBX transformed into yeast strain Y187 to test if the bait alone led to transcriptional activation. α-galactosidase assay screening indicate negative interaction between bait and library. Colonies remained white. F3 and L8: control using negative control plasmid, pGBKT7-Lamin transformed into yeast strain Y187. α-galactosidase assay screening indicate negative interaction between bait and library. Colonies remained white. F4 and L9: Control using bait plasmid, pGBKT7 transformed into yeast strain Y187. α-galactosidase assay screening indicate negative interaction between bait and library. Colonies remained white.Back to article page