Figure 2From: Neutrophil elastase, an acid-independent serine protease, facilitates reovirus uncoating and infection in U937 promonocyte cellsEffects of agents that raise vesicular pH on reovirus replication in U937 and L929 cells. A. U937 cells were pre-treated without (-; black bars) or with 300 μM E64 (E64; grey bars) in the presence or absence of 25 nM Baf or 20 mM NH4Cl. Following pre-treatment, cells were infected with reovirus strain Lang at an MOI of 3 and viral yield was measured at 3 d.p.i as described in the legend to Fig. 1B. B. L929 cells were pre-treated without (-) or with E64, 25 nM Baf or 20 mM NH4Cl. Pre-treated cells were infected with reovirus strain Lang at an MOI of 3 and viral yield was measured at 3 d p.i. as described in the legend to Fig. 1B.Back to article page