Figure 1From: Transmission of human hepatitis C virus from patients in secondary cells for long term cultureIsolation of HCV from human patients. (A) Isolation scheme for the replication of HCV in vitro. (B) History of transmission of the specimen donated from HCV infected patient #081. Fresh macrophages were infected by using cell-free serum or cocultured with HCV infected PBMC from the blood of patient #081. Human T-cells (112 A), B-cells (112 B) or the non-committed lymphoid cells (112 AB) were then either infected by cell-free transmission of HCV from cell culture supernatant from macrophages or cocultured with HCV infected macrophages. Similarly freshly transformed cord blood B-cells (PCLB 1°) were infected by cell free transmission from previously infected B-cell (112 B) culture supernatant. Uninfected transformed B-cells (PCLB T1-T4) were infected by serial, cell-free transmission from filtered PCLB 1° culture supernatant. Neuronal precursor cells were infected by cell free transmission of HCV from filtered #081 culture supernatant.Back to article page