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Figure 2 | Virology Journal

Figure 2

From: Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture

Figure 2

Monitoring of wt and recS-RNA during sequential passages of the mixture of TUL02 and recTULV. A. PCR-amplicons (118 bp), obtained in RT- PCR with the primers VF738 and VR855 (specific for the wt virus) on RNA from infected cells collected on passages 1 to 10. B. PCR-amplicons (118 bp), obtained in RT- PCR with the primers RECF738 and RECR855 (specific for the recombinant virus) on RNA from infected cells collected on passages 1 to 10. NC, negative controls. M, molecular weight markers; bands of 147 and 110 bp are indicated by arrows.

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Virology Journal

ISSN: 1743-422X