Figure 6
Levels of HCV RNA as determined by Northern analysis. Cells (n = 1 × 106) were either mock-infected (lanes 1, 6 and 11) or infected with JFH1 (MOI = 5) and harvested at 48 h after infection. Northern analysis was carried out with RNA isolated from Huh7.5.1 cells transfected with M1GS-HCV/C67* (lanes 3, 8 and 13), M1GS-HCV/C67 (lanes 4, 9 and 14), and Chol-M1GS-HCV/C67 (lanes 5, 10 and 15). Equal amount of each RNA sample was separated on agarose gels containing formaldehyde, transferred to a nitrocellulose membrane, and hybridized to a 32P-radiolabeled probe containing the cDNA sequences of HCV core coding region (A), M1 RNA gene (B), and human β-actin gene (C), respectively.