Figure 2

Identification of the recovered Newcastle disease viruses. (A) The genetic markers of the rescued NDV-D90 were confirmed by sequencing. The Kpn I restriction enzyme site in the L gene was eliminated by site-directed mutagenesis (A13996T) as the genetic marker. (B) The rescued rNDV-D90 infected BSR-T7/5 or CEF cells at an MOI of 0.01. After 48 h, the expressions of viral proteins were confirmed by IFA. Uninfected cells served as the negative control. (C) EGFP expression in rNDV-GFP-infected BSR-T7/5 or CEF cells. BST-T7/5 or CEF cells were infected with rNDV-GFP at a MOI of 0.01. Cells were observed at 24 h and 48 h post-infection.