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Table 2 Real-time PCR to verify expression pattern of differentially expressed genes from the microarray experiment (for a list of these genes primer sequence, see Additional file 2 : Table S2)

From: Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation

GB.accession

Fold change (Ex/Mock) ± SD

p-valueB

p-valueA

Description

Microarray

qRT-PCR

AK069685

5.4983 ± 1.581

6.6246 ± 3.6546

0

0.0204

Piwi domain containing protein, expressed

9631.m02900

2.6141 ± 0.7001

2.9988 ± 0.530

0

0.0316

Small nuclear ribonucleoprotein G, putative, expressed

AF443600

6.2009 ± 2.7810

6.5142 ± 2.1441

0.0042

0.0045

Glucan endo-1,3-beta-glucosidase GII precursor, putative, expressed

AF247164

6.1081 ± 6.8408

6.7585 ± 11.4824

0.0236

0.0235

Alpha-expansin 4 precursor, putative, expressed

AK099501

2.1007 ± 0.9030

2.4711 ± 0.5329

0.0261

0.005

Ribonucleoprotein, putative, expressed

CR282531

1.7015 ± 0.2375

1.4174 ± 0.5866

0.0303

0.0694

60S acidic ribosomal protein P2A, putative, expressed

AK103199

3.2899 ± 2.5965

4.0319 ± 3.4615

0.0302

0.0057

Transposon protein, putative, CACTA, En/Spm sub-class, expressed

AK062099

1.6257 ± 0.3806

1.7461 ± 0.3890

0.0308

0.0601

Ribosomal L28e protein family protein, expressed

AK059679

1.8421 ± 0.303

1.9076 ± 0.9539

0.0308

0.0892

60S ribosomal protein L38, putative, expressed

AK067896

1.6171 ± 0.2825

1.3415 ± 0.6274

0.04342

0.1508

60S ribosomal protein L6, putative, expressed

AK120766

1.7459 ± 0.5397

2.1115 ± 0.8514

0.04342

0.0293

Piwi domain containing protein, expressed

D29724

1.529 ± 0.2032

1.51084 ± 0.2562

0.04342

0.0408

Peptide chain release factor 2, putative, expressed, 60S ribosomal protein L38, putative, expressed

AK063247

2.5979 ± 1.4057

2.0925 ± 2.0497

0.04342

0.0197

Auxin-induced protein TGSAUR12, putative, expressed

AK062943

1.5919 ± 0.0932

1.5679 ± 1.0226

0.0483

0.0986

40S ribosomal protein S15a, putative, expressed

AK071291

1.6782 ± 0.3353

1.5959 ± 0.6893

0.0483

0.0831

Fibrillarin-2, putative, expressed

AK061513

2.5152 ± 0.6761

2.8184 ± 1.9757

0.0588

0.0434

Nucleoid DNA-binding protein cnd41, putative, expressed

AK099754

1.7647 ± 0.2432

1.7471 ± 0.2758

0.0588

0.0487

Nucleolar protein NOP5, putative, expressed

U36565

–

4.8787 ± 2.0832

–

7.23E-06

Rice dwarf virus coat protein mRNA, complete cds

UBQ11*

–

–

–

0.1955

 
  1. Notice: Ap-value form microarray experiment; Bp-value from qRT-PCR; *Rice UBQ11 was used as a control for qRT-PCR.