Molecular characterisation of human hepatitis E virus from Italy: comparative analysis of five reverse transcription-PCR assays

Table 2 PCR results using five different nested assays

Patient code	Real-Time PCR^a	RT-PCR/nestedPCR					Country of Origin^b
Patient code	Real-Time PCR^a	Method A (ORF1, MTase)	Method B (ORF1, RdRp)	Method C (ORF2, capsid)	Method D (ORF2, capsid)	Method E (ORF2/ORF3)	Country of Origin^b
8	+ + +	Genotype 1	Genotype 1	Genotype 1a	Genotype 1a	Genotype 1	Italy (India)
10	+	-		-	-	-	Ethiopia
12	+ + +	-		-	-	Genotype 1	India
16	+ +	Genotype 1		Genotype 1a	Genotype 1a	Genotype 1	Bangladesh
19	+ +	-		-	-	-	Bangladesh
22	+ +	-	Genotype 1	Genotype 1a	Genotype 1a	Genotype 1	Italy (India)
29	+	-		-	-	-	Bangladesh
33	+	-		-	-	-	Italy
34	+	-		-	-	-	Italy
43	+ +	Genotype 1		-	-	-	Bangladesh
47	+ +	-		-	-	-	Bangladesh
48	+ +	-	Genotype 1	-	-	-	Bangladesh
51	+ +	-		-	-	-	Italy
52	+	Genotype 1		Genotype 1a	-	-	Bangladesh
55	+ +	Genotype 1		-	-	-	India
56	+ +	-		-	-	-	Bangladesh
58	+ +	Genotype 1		-	-	-	Italy (Thailand)
61	+ +	-		-	-	-	Italy (India)
63	+ + +	-		-	-	-	Albania
69	+	Genotype 3		-	-	Genotype 3	Italy
71	+	-		-	-	-	Bangladesh
81	+	-		-	-	-	Italy
86	+	-		-	-	-	Italy
92	+	Genotype 3		-	-	-	Italy

^a+ : 250 < copies/mL < 2,500; + + : 2,500 < copies/mL <25,000; + + +: >25,000 copies/mL.
^bTravel to/from country of origin for foreign patients who travelled abroad in the last month. Travel to countries from endemic areas are indicated in brackets for Italian patients.

ISSN: 1743-422X