Figure 2From: Activation of the PKR/eIF2α signaling cascade inhibits replication of Newcastle disease virusDetection of dsRNA in NDV infected cells. HeLa cells grown on glass coverslips were infected with NDV strain LaSota or Herts/33 at an MOI of 1 or treated with UV-inactivated Herts/33 for 12 h. dsRNAs were detected by immunofluorescenct staining with the specific anti-dsRNA monoclonal antibody J2. The secondary antibody was Cy3-labeled goat anti-mouse IgG (red). Nuclei were stained with 1 μg/mL of DAPI.Back to article page