Figure 3

GA and 17-AAG decreased the level of viral N protein. PRRSV-infected cells were treated with chemicals at the concentration as indicated. Mock-infected cells were untreated. (A) MARC-145 cells and (B) PAMs were harvested at 24 h.p.i for western blotting analysis. The level of viral N protein was quantified by measuring band intensities and normalized with respect to the amount of β-actin. Data are mean ± SD, n = 3, and significant differences compared with DMSO (in MARC-145 cells) or no drug (in PAMs) treatment group, respectively, are denoted by ^*(P < 0.05). (C) Inhibitors-treated MARC-145 cells were fixed at 24 h.p.i to detect viral N protein (red) by IFA. Nuclei were stained with Hoechst dye 33258 (blue). Bar, 200 μm.