Table 1 Primer sequences and annealing temperatures used to sequence the genome of H3N8 EIV
Primer name | Primer sequence (5′-3′) | Approximate nucleotide coverage (5′-3′) | Annealing temperature used (°C) |
|---|---|---|---|
PB2/AF | GC GTAAAACGACGGCCAGT AGCGAAAGCAGGCAAATATATTCAATATG | 1-655 | 50 |
PB2/AR | GC AACAGCTATGACCATG CTCTTTCTAGCATGTAT | ||
PB2/BF | GC GTAAAACGACGGCCAGT CACAACTAACAATAACCAA | 569-1335 | 60 |
PB2/BR | GC AACAGCTATGACCATG CCTCAAGAGTTGATG | ||
PB2/CF | GC GTAAAACGACGGCCAGT GCAATAATTGTAGCC | 1216-1874 | 45 |
PB2/CR | GC AACAGCTATGACCATG ATTATTTGAGCAGTATC | ||
PB2/DF | GC GTAAAACGACGGCCAGT GAAGCCAATACAGCGGT | 1793-2341 | 50 |
PB2/DR | GC AACAGCTATGACCATG AGTAGAAACAAGG TCGTTTTTAAACAATTC | ||
PB1/AF | GC GTAAAACGACGGCCAGT AGCGAAAGCAGG CAAACCATTTGAATGG | 1-719 | 50 |
PB1/AR | GC AACAGCTATGACCATG CAGCGTCCTTGGTCATTG | ||
PB1/BF | GC GTAAAACGACGGCCAGT CTTCCAACGAAAGAGAA | 577-1301 | 50 |
PB1/BR | GC AACAGCTATGACCATG GGTTTAATATGGATACACC | ||
PB1/CF | GC GTAAAACGACGGCCAGT GCGGCTTCACTGAGTCCTGGC | 1222-1863 | 50 |
PB1/CR | GC AACAGCTATGACCATG CATTTTAAACAAACTTC | ||
PB1/DF | GC GTAAAACGACGGCCAGT CAAAGACTGGTCTACTG | 1789-2341 | 50 |
PB1/DR | GC AACAGCTATGACCATG AGTAGAAACAAGG CATTTTTTCATGAAGATC | ||
PA/AF | GC GTAAAACGACGGCCAGT AGCAAAAGCAGG TACTGATCCAAAATGG | 1-615 | 50 |
PA/AR | GC AACAGCTATGACCATG GCCTCTCTCGGACTGAC | ||
PA/BF | GC GTAAAACGACGGCCAGT GCCAGAATCAAGACCAGG | 529-1255 | 50 |
PA/BR | GC AACAGCTATGACCATG CTCACTTGGAATCCAACTTGC | ||
PA/CF | GC GTAAAACGACGGCCAGT GAGAGAAAGTGGATTTTGAGGATTG | 1149-1785 | 50 |
PA/CR | GC AACAGCTATGACCATG CTGAAGGAGGCAGCGCC | ||
PA/DF | GC GTAAAACGACGGCCAGT GACCCATGTTTTTGTATG | 1700-2233 | 50 |
PA/DR | GC AACAGCTATGACCATG AGTAGAAACAAGG TACTTTTTTGGACAG | ||
HA/AF | GC GTAAAACGACGGCCAGT AGCGAAAGCAGGGGACGATATT | 1-515 | 50 |
HA/AR | GC AACAGCTATGACCATG GATTTGTTAGCCAATTCAG | ||
HA/BF | GC GAAAACGACGGCCAGT CAGGTGTCACTCAAAAC G | 428-1032 | 50 |
HA/BR | GC AACAGCTATGACCATG GGATTTGCTTTTCTGGTAC | ||
HA/CF | GC GTAAAACGACGGCCAGT GGTTACATATGGAAAATGCC | 939-1336 | 50 |
HA/CR | GC AACAGCTATGACCATG GAGCCACCAGCAATTCT | ||
HA/DF | GC GTAAAACGACGGCCAGT GAAGGAAGAATTCAGGA | 1251-1733 | 50 |
HA/DR | GC AACAGCTATGACCATG GAGTAGAAACAAGGGTGTTTTTAAC | ||
NP/AF | GC GTAAAACGACGGCCAGT AGCGAAAGCAGGGTAGATAATC | 1-570 | 50 |
NP/AR | GC AACAGCTATGACCATG CCGTGGGAGGGTTGAGCC | ||
NP/BF | GC GTAAAACGACGGCCAGT GACACCACATACCAAAC | 480-1075 | 45 |
NP/BR | GC AACAGCTATGACCATG CTCTCAGGTCCTCAAAT | ||
NP/CF | GC GTAAAACGACGGCCAGT CCAGCACACAAGAGCCAG | 1012-1569 | 55 |
NP/CR | GC AACAGCTATGACCATG AGTAGAAACAAGGGTATTTTTC | ||
NA/AF | GC GTAAAACGACGGCCAGT AGCAAAAGCAGGAGTTT | 1-508 | 45 |
NA/AR | GC AACAGCTATGACCATG GCCCTATTTTGACACTC | ||
NA/BF | GC GTAAAACGACGGCCAGT CACACAGGGCTCATTAC | 417-1049 | 45 |
NA/BR | GC AACAGCTATGACCATG CCGAAACCTTTTACACCG | ||
NA/CF | GC GTAAAACGACGGCCAGT CACAGTTGGATATTTGTG | 951-1461 | 50 |
NA/CR | GC AACAGCTATGACCATG AGTAGAAACAAGGAGTT | ||
M/AF | GC GTAAAACGACGGCCAGT AGCGAAAGCAGGTAGATATTTAAAG | 1-654 | 50 |
M/AR | GC AACAGCTATGACCATG CTAGCCTTACTAGCAAC | ||
M/BF | GC GTAAAACGACGGCCAGT CAGTACCACGGCTAAAG | 571-1027 | 50 |
M/BR | GC AACAGCTATGACCATG AGTAGAAACAAGGTAGTTTTTTAC | ||
NS/AF | GC GTAAAACGACGGCCAGT AGCGAAAGCAGGGTGACAAAAAC | 1-492 | 50 |
NS/AR | GC AACAGCTATGACCATG CTGCTCCTTCTTCGGTG | ||
NS/BF | GC GTAAAACGACGGCCAGT CATCATACTTAAAGCAAAC | 407-890 | 50 |
NS/BR | GC AACAGCTATGACCATG AGTAGAAACAAGGTAGTGTTTTTTAT |