Table 7 Primers and TaqMan MGB probes used for detection of aa 70 substitutions
From: Quantitation of substitutions at amino acid 70 in hepatitis C virus genotype 1b
Type | Primer name | Binding position (nt) | Sequence (5′–3′) |
|---|---|---|---|
Primers | sense | 148–167 | AGG AAG ACT TCC GAG CGG TC |
antisense | 302–286 | CGG GGT GAC AGG AGC CA | |
Degenerate probes | 70 W(CGN) | 203–217 | FAM-CTC GCC GIC CCG AGG-MGB |
70 M(CAN) | 203–219 | VIC-CTC GCC AIC CCG AGG GC-MGB | |
Normal probes | 70 W(CGG) | 204–217 | FAM-TCG CCG GCC CGA GG-MGB |
70 M(CAG) | 203–218 | VIC-CTC GCC AGC CCG AGG G-MGB |