Figure 1

Identification of pRSV, pRSVΔG-EGFP and four helper plasmids. (A) Restriction patterns of cDNA clones of pRSV, pRSV∆G-EGFP. M, DNA Ladder DL15000; 1, pRSV was digested with sal I and kpn I (length 15222 bp, 4108 bp); 2, pRSV∆G-EGFP was also digested with sal I and kpn I (length 15050 bp, 4108 bp); (B) Restriction patterns of pCN-N and pCI-P helper plasmids. 3, plasmid pCI-N was digested with EcoRI and KpnI (length 1176 bp, 4006 bp); 4, plasmid pCI-P was digested with NheI and KpnI (length 726 bp, 4006 bp); (C) Restriction patterns of pCN-L and pCI-M2-1 helper plasmids. 5, plasmid pCI-L was digested with XhoI and SalI (length 6498 bp, 4006 bp); 6, plasmid pCI-M2-1 was digested with XhoI and XbaI (length 585 bp, 4006 bp).