Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Table 4 Detection of MERS-CoV diluted with medium containing pharyngeal swabs

Copies/50 μL	500,000	50,000	5000	500	50	5	0.5	Negative control	Sensitivity (copies)	Time required
Virus isolation*
Vero	6/6	6/6	6/6	3/6	0/6	0/6	0/6	0/6	500	5 d
Vero/TMPRSS2	6/6	6/6	6/6	6/6	5/6	0/6	0/6	0/6	23.2	2 d
Copies/reaction	50,000	5000	500	50	5	0.5	0.05
Real-time RT-PCR**
upE	22.0	25.3	28.7	32.3	33.2	> 40	> 40	> 40	1.6	2 hr
ORF1a	21.9	25.2	28.5	32.2	32.5	> 40	> 40	> 40	1.6	2 hr
RT-LAMP***	11.00	11.16	12.18	13.48	18.04	23.12	> 30	> 30	0.7	30 min

ISSN: 1743-422X