Table 1 Primers used to amplify and sequence the complete BATV S, M, and L segments
From: Isolation and complete nucleotide sequence of a Batai virus strain in Inner Mongolia, China
Segment primer | Sequence (5′ to 3′) | Amplicon size (nt) |
|---|---|---|
S segment primer | Â | Â |
BATAS1F | 5′-GAATTCAATGATGTCGCTGCT-3′ | 818 |
BATAS1R | 5′-CAATTTGCTGTGCTCTTTCTG-3′ | |
3′S-Inner Primer | 5′-CTAGCAAAGTTTGGCATCAGC-3′ |  |
3′S-Outer Primer | 5′-GGGTGGAAGAAGACAAATGTG-3′ | |
5′S-Inner Primer | 5′-GGGTCAAAAGTACTGCTGGTG-3′ | |
5′S-Outer Primer | 5′-GTGTAGATACGCTTAAAGTTA-3′ | |
M segment primer | Â | Â |
BATAM1F | 5′-GATGTTGCTACTTCTTGTCTT-3′ | 2013 |
BATAM1R | 5′-AGTTTTGTAATACCAGTTGTG-3′ | |
BATAM2F | 5′-CAACTGAGTCACTCTTTTATG-3′ | 1973 |
BATAM2R | 5′-CATAATCTTGTTTTGTGGAGG-3′ | |
BATAM3F | 5′-CTGCAGTTTTAAGATTGTTTC-3′ | 480 |
BATAM3R | 5′-TGTTTTATTTCCTGTAGGTAC-3′ | |
3′M-Inner Primer | 5′-GTACCTACAGGAAATAAAACA-3′ |  |
3′M-Outer Primer | 5′-TGATGTTTAAACTTAGAGATG-3′ | |
5′M-Inner Primer | 5′-GAGGCAAAATTCTGATATAGC-3 | |
5′M-Outer Primer | 5′-ATTTTTTTCATATATTATTTC-3′ |  |
L segment primer | Â | Â |
BATAL1F | 5′-AAAAATGGATGATCAGATGTA-3′ | 2025 |
BATAL1R | 5′-TTCTCTGCTATATAATCTTTG-3′ | |
BATAL2F | 5′-TCGATATATGATAATGAATTC-3′ | 2021 |
BATAL2R | 5′-CTAAACCAACTAAAGCTATCA-3′ | |
BATAL3F | 5′-TTAAGAGTCGTCATGATATAC-3′ | 1952 |
BATAL3R | 5′-TGATAATACAACAGGACAGAC-3′ | |
BATAL4F | 5′-TAAAAAGAAATGAAGAAGGAC-3′ | 945 |
BATAL4R | 5′-CTTAGAAAAAGGTGAACATGG-3′ | |
3′L-Inner Primer | 5′-CAACTGGACCAAGATGCTAAA-3′ |  |
3′L-Outer Primer | 5′-CTGCCTTCTACATAAATACAG-3′ |  |
5′L-Inner Primer | 5′-GTTCCAACTGATACTTTATAG-3′ |  |
5′L-Outer Primer | 5′-GTATTTTTTATAAGTAATCTC-3′ |  |
P1 | 5′-ATAGAATCAGCAATAGCAAGC-3′ | 550 |
P2 | 5′-ATGATGATCTGTAACCTCTAA-3′ |