Table 1 Speed-optimized extraction protocols for the KF Duo and the BS 15 instrument
From: Development and validation of rapid magnetic particle based extraction protocols
Step | Reagents | Timing of protocol steps (min) | ||
|---|---|---|---|---|
Original | KF Duo | BS 15 | ||
Binding | VXL + ACB + Beads | 5 | 2 | 2 |
Wash 1 | AW1 | 3 | 12 s | 1 |
Wash 2 | AW2 | 2 | 12 s | 1 |
Wash 3 | Ethanol 100% | 2 | 12 s | - |
Evaporation | Water | 5 | 0 | 0 |
Elution | AVE | 2 | 1 | 0.5 |
Total time | 24:00 | 8:00 | 7:50 | |