Skip to main content
Figure 4 | Virology Journal

Figure 4

From: Effects of two amino acid substitutions in the capsid proteins on the interaction of two cell-adapted PanAsia-1 strains of foot-and-mouth disease virus serotype O with heparan sulfate receptor

Figure 4

Analysis of the interaction of the indicated FMDVs with the cell surface HS of WT-CHO cells by IFA. In virus adsorption assay, WT-CHO cells were inoculated with O/Fujian/CHA/9/99wt and pgsD-677 cells were inoculated with each of the indicated virus as a negative control and references for non-HS binding, respectively. BHK-21 cells infected with O/Fujian/CHA/9/99wt were used as a parallel comparison (positive controls) in virus adsorption and penetration assays. Only the merged photomicrographs are shown (bar = 10 μm). The HS-binding of O/Tibet/CHA/6/99tc, rHN, rHN/FJ9-VP0 and rHN/TAR6-VP0Q2080L (red) and the synthesis of 3A protein of O/Tibet/CHA/6/99tc (green) were viewed by confocal microscope.

Back to article page

Virology Journal

ISSN: 1743-422X