Figure 5From: Mutations within the conserved NS1 nuclear export signal lead to inhibition of influenza A virus replicationExpression kinetics of viral proteins during infection. A549 cells were infected at MOI 1 with A/Udorn/72 wild type and mutant viruses and cell lysates were collected at the indicated time points. The amounts of NS1, NP, NEP, M1 and actin in the lysates were visualized by immunoblotting (10Â ÎĽg total protein/lane). Data shown is representative of three independent experiments.Back to article page