Figure 5

Time course of immunofluorescent staining of virus infected adult lungs. Groups of 2 adult mice were infected intranasaly with 10⁷ pfu of either T1L or T3D before fluorescent antibody staining of frozen lung sections at 1, 2, or 3, days pi. T1L and T3D antigens were stained with virus specific antiserum as the primary antibody followed by CY3 labeled donkey anti-rabbit IgG and are colored in red. Cell nuclei were stained with DAPI and are shown in blue, either alone or merged with the CY3 stained images. Sagital sections show extensive staining in alveolar regions as well as sparse staining of epithelium in the bronchiolar airways (arrow) restricted primarily to T3D infection. Uninfected control lungs are shown as negative staining controls. Size bars are 100 μM.