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Table 4 Test of sensitivity of Pan-Flavi assay with pre-quantified viral RNA dilutions

From: Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses

Quantity (Genome copies/reaction)

Ct values for DENV-1

Ct values for TBEV strain K23

Ct values for YFV strain 17D

1 × 107

16.8

16.6

-

1 × 106

20.5

19.5

20.8

1 × 105

23.4

23.0

24.2

1 × 104

27.4

26.2

27.6

1 × 103

30.2

29.4

30.1

1 × 102

37.1

31.7

36.4

1 × 101

39.7

33.0

No Ct

  1. GC: genome copies, Ct: threshold cycle.
  2. YFV: Yellow fever virus, TBE: Tick-borne encephalitis virus, DENV: Dengue virus.
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Virology Journal

ISSN: 1743-422X