Table 2 Primers used in this study
From: Characterization of an envelope gene VP19 from Singapore grouper iridovirus
Primer name | Primer sequence (5′-3′) |
|---|---|
pET-VP19t-F | CGGAATTCATGGCATCGTCCACTATAC |
pET-VP19t-R | GCCAAGCTTAATACTTTGAAAAATCGC |
EGFP-VP19-F | CGCAAGCTTCGATGGCATCGTCCACTATAC |
EGFP-VP19-R | AGTGGATCCCCCAAAAGAGCCCTTCATT |
VP19-F | TCCAAGGGAGAAACTGTAAG |
VP19-R | GGGGTAAGCGTGAAGACT |
MCP-F | GCACGCTTCTCTCACCTTCA |
MCP-R | AACGGCAACGGGAGCACTA |
Actin-F | TACGAGCTGCCTGACGGACA |
Actin-R | GGCTGTGATCTCCTTCTGCA |