Table 2 Sequences of primers and PCR conditions

Reverse transcription

5′-TGR TGC ACG GTC TAC GAG ACC TC-3′

Temp. 72°C/30 min

I PCR

5′-RAYCACTCCCCTGTGAGGAAC-3′

1 min at 94°C;

50 cycles of 94°C for 15 s, 58°C for 30 s, and 72°C for 30 s; 72°C for 7 min

II PCR

5′-ACTGTCTTCACGCAGAAAGCGTC-3′

1 min at 94°C;

5′-CAAGCACCCTATCAGGCAGTACC-3′

30 cycles of 94°C for 15 s, 58°C for 1 min, and 72°C for 1 min; at 72°C for 7 min