Table 3 Alignment of amino acid sequences in the highly polymorphic region (HPR) of the HE genes from selected low pathogenic infectious salmon anaemia virus (ISAV-HPR0) and virulent infectious salmon anaemia virus (ISAV-HPR∆)

HPRO

United Kingdom NWM10 (FJ178189)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

N

T

N

Q

V

E

Q

P

S

T

S

V

L

S

N

I

F

I

S

M

G

V

A

0

HPRO

Scotland 1703/01/86 (AJ440971)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

N

T

N

Q

V

E

Q

P

A

N

S

V

L

S

N

I

F

I

S

M

G

V

A

0

HPRO

Chile CH29/o8 (JN711094)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

N

T

N

Q

V

E

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

0

HPR20

Canada RPC/NB 98-0280-2 (AF294870)

T

D

V

K

I

R

V

D

A

I

-

-

-

L

-

-

-

-

G

V

N

Q

V

E

Q

P

S

T

S

V

L

S

N

I

F

I

S

M

G

V

A

7

HPR36

Norway Vir22 (DQ785258)

T

D

V

K

I

R

V

D

A

-

-

-

-

-

-

-

-

F

N

T

N

Q

V

E

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

8

HPR6

Norway MR60/01 (AY127876)

T

D

V

K

I

R

V

D

A

I

-

-

-

-

-

-

-

-

-

-

-

Q

V

E

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

11

HPR14

Norway ST21/96 (AF364886)

T

D

V

K

I

R

V

D

A

-

-

-

-

-

-

-

-

-

-

-

N

Q

V

E

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

11

HPR33

Norway N127/07 (JN711066)

T

D

V

K

I

R

V

D

-

-

-

-

-

-

-

-

-

-

-

D

N

-

V

E

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

12

HPR17

Norway MR103/05 (DQ108606)

T

D

V

K

I

R

-

-

-

-

-

-

-

L

-

-

-

-

-

-

-

E

V

E

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

14

HPR9b

Chile 2006B-13364 (FJ594284)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

N

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

I

S

M

G

V

A

15

HPR12

Norway N5/89 (AY127882)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

N

I

F

I

S

M

G

V

A

15

HPR21

Chile 7833–1 (AF294879)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

S

N

I

F

I

S

M

G

V

A

17

HPR3

Norway ST110/05 (DQ108598)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

F

I

S

M

G

V

A

17

HPR4

Norway Gullesfjord/94 (AF302801)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

S

N

I

F

I

S

M

G

V

A

17

HPR4c

Chile 26560–10 (EU625666)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

N

I

F

I

S

M

G

V

A

18

HPR9

Norway FM116/06 (JN711058)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

N

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

G

V

A

18

HPR10

Norway MR52/00 (AF364892)

T

D

V

K

I

R

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

18

HPR12a

Norway ISAV3 (DQ785247)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

N

I

F

I

S

M

G

V

A

18

HPR31

Norway T126/07 (JN711076)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

I

F

I

S

M

G

V

A

18

HPR11

Norway SF57/00 (AF364890)

T

D

V

K

I

R

V

D

A

I

P

P

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

R

N

I

F

I

S

M

G

V

A

19

HPR13

Faroe Island 1173/01/12 (AJ440970)

T

D

V

K

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

E

Q

P

A

N

S

V

L

S

N

I

F

I

S

M

G

V

A

19

HPR16

Norway T90/04 (AY971666)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

M

G

V

A

19

HPR21b

Canada NB1330-2 (AY646063)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

M

G

V

A

20

HPR35

Norway NT156/09 (JN711072)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

F

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

G

V

A

20

HPR2

Norway T131/07 (JN711078)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

M

G

V

A

20

HPR19

Norway SF18/96 (AF364869)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

N

Q

T

-

-

-

-

-

-

-

-

-

-

-

-

-

L

-

-

-

-

-

-

-

G

V

A

20

HPR1

Norway FM86/04 (AY971659)

T

D

V

K

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

21

HPR5

Norway T152/09 (JN711086)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

I

S

M

G

V

A

21

HPR34

Norway NT134/08 (JN711069)

T

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Q

P

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

21

HPR1c

Chile 31606-H (FJ594281)

T

D

V

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

23

HPR7b

Chile 31991-3N (FJ786983)

T

D

V

K

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

T

S

V

L

S

N

I

F

I

S

M

G

V

A

23

HPR15

Norway H56/00 (AF364880)

T

D

V

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

E

-

-

-

T

S

V

L

S

N

I

F

I

S

M

G

V

A

23

HPR15c

Chile 901 (GU830908)

T

D

V

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

A

T

S

V

L

S

N

I

F

I

S

M

G

V

A

23

HPR15e

32246 (FJ86988)

T

D

V

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

T

-

-

-

-

-

-

-

T

S

V

L

S

N

I

F

I

S

M

G

V

A

23

HPR8

Norway MR46/99 (AF364896)

T

D

V

K

I

R

V

D

A

I

P

P

Q

L

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

G

V

A

24

HPR15b

cHILE 31150–3 (FJ786985)

T

D

V

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

T

S

V

L

S

N

I

F

I

S

M

G

V

A

24