Figure 6
From: The anti-HBV effect mediated by a novel recombinant eukaryotic expression vector for IFN-α
Lamivudine combined with pSecTagB-IFN-α displayed enhanced anti-HBV effect than with exogenously supplemented IFN-α. (A-C) HepG2.2.15 cells were transfected with pSecTagB-IFN-α and treated with lamivudine (3 μmol/L), or treated with IFN-α2a (30 IU/mL) and lamivudine (3 μmol/L). Then, RNAs were extracted after 24 h and 48 h. The relative mRNA expression of HBx, HBs and HBc were examined by qRT-PCR. (D) The level of HBV DNA was quantified by fluorescence real-time PCR. (E, F) The contents of HBsAg and HBeAg were detected by ELISA. Data are expressed as the mean ± SD from at least three independent experiments. *p < 0.05: versus the combination of exogenous IFN-α2a and lamivudine stimulated group.