Figure 5

Reverse transcriptase PCR (RT-PCR). Whole cell RNA from infected lung tissue on days 3, 5, 7 and 10 after inoculation was isolated, DNase-treated, and converted into cDNA using the reverse transcriptase enzyme. The resulting cDNA was amplified using RSV-A2 specific primers. The distinct band of correct size (334 bp, with intensity peaking on day 5) is absent in the uninfected lung, but present in infected lung and in the cDNA sample from purified RSV virions. The same results were obtained in 2 independent experiments.