Figure 1
Comparison of the in vitro and in vivo replication competence of pBS-HBV1. 1 B , pBS-HBV1.2 B and pBS-HBV1.3 B . 0.8 μg of plasmid DNA were transfected into Huh-7 cells which were seeded in 24-well plates at approximately 60% confluence and 10 μg of plasmid DNA were injected into the tail veins of BALB/c mice. Each group included 10 mice. (A) Titers of HBsAg (ng/ml) and HBeAg (NCU/ml, National Clinical Unit/ml,) in the supernatants of pBS-HBV1.1B, pBS-HBV1.2B and pBS-HBV1.3B transfected Huh-7 cells at 48 h post-transfection. The data were analyzed by one-way ANOVA, and the differences were statistically significant (* and Δ mean p < 0.01). (B) Real-time PCR detection of HBV DNA in mouse sera at day 7 after HI of pBS-HBV1.1B, pBS-HBV1.2B and pBS-HBV1.3B (the number of the mice ≥ 3). The data were analyzed by one-way ANOVA, and the differences were statistically significant (* means p < 0.01). (C) Immunohistochemical staining of the liver sections for HBcAg in hepatocytes of pBS-HBV1.1B- (1), pBS-HBV1.2B- (2), pBS-HBV1.3B- (3) and pBS (4)-injected mice at 7 day post injection (Original magnification: 200X).