Test performed | Cell linef | ||||||||
---|---|---|---|---|---|---|---|---|---|
 | CV-1 | HEK-293 | LLC-MK2 | MDCK | MDCK-London | Mv1 Lu | RPTEC | Vero E6 | WI-38 |
Microscopy, 12Â hr post-seed | NAa | NA | NA | NA | NA | NA | Owl eye nuclei; enlarged cells | NA | NA |
IFA, 48Â hr post-seed | NA | NA | NA | NA | NA | NA | CMV positive | NA | NA |
PCR, 48Â hr post-seed | NA | NA | NA | NA | NA | NA | CMV positiveg | NA | NA |
BGM cytotoxicity | No effect | NTc | No effect | No effect | NT | NT | NA | No effect | No effect |
Bioactive agent release assay | Vacuolation 12 hpi, 37°C | Vacuolation 12 hpi, 37°C | Vacuolation 12 hpi, 37°C | NT | NT | NT | NA | Vacuolation 12 hpi, 37°C | C.R., Sw. & Vac.h 12 hpi, 37°C; cell death 48 hpi |
Subcultures, 5 d post-seed of RPTEC | Vacuolation 12 hpi; CPE 6 dpib | CPE 7 dpid | Vacuolation 12 hpi; CPE 5 dpie | CPE 6 dpie | CPE 6 dpie | CPE 6 dpie | NA | Vacuolation 12 hpi; CPE 6 dpib | C.R. Sw. & vac.h 12 hpi, 37°C; cell death 48 hpi |
Subcultures, freeze-thaw 7d post-seed of RPTEC | CPE 6 dpib | CPE 7 dpid | CPE 5 dpie | CPE 6 dpie | CPE 6 dpie | CPE 5 dpie | NA | CPE 6 dpie | No CPE 30 dpi |