Figure 3

Dose response of ribavirin. HEp-2 cells were plated in 384-well black tissue culture plates at 2,000 cells per well. Ribavirin or carrier control (DMSO) were diluted to 6x in Complete DMEM/F12 and serially diluted 1:2 resulting in an 8 point dose response dilution series. (final plate well concentration ranging from 50 uM to 0.39 uM and a final DMSO concentration of 0.5%). For dose response screening, cells were infected with a 1:500 dilution of virus (viral MOI = 0.5). The assay plates were incubated for six days at 37°C, 5% CO₂ and 90% relative humidity and the inhibitory effects of the drug were assessed using Cell Titer Glo. The EC₅₀ value (squares) was performed in triplicate while the IC₅₀ value (triangles) was performed in duplicate.