Virology Journal

Table 1 Detail of samples collected from districts and diagnostic efficacy of real-time PCR

From: Genetic diversity of Newcastle disease virus in Pakistan: a countrywide perspective

Province	District	Type of birds
		¹Commercial				²Rural				³Wild captive
		Flock (n)	Blood samples (n)	⁴Real time PCR		Flock (n)	Blood samples (n)	Real Time PCR		Flock (n)	Blood samples (n)	Real time PCR
				M gene	F gene			M gene	F gene			M gene	F gene
Baluchistan	Quetta	09	36	+	+	03	12	+	+	0.0	0.0	-	-
	Noshki	0.0	0.0	-	-	04	16	-	-	0.0	0.0	-	-
	Zhob	0.0	0.0	-	-	01	04	-	-	0.0	0.0	-	-
	Mastoung	0.0	0.0	-	-	02	08	-	-	0.0	0.0	-	-
	Loralai	09	03	+	+	05	20	+	+	0.0	0.0	-	-
KPK	Abbottabad	11	44	+	+	0.0	0.0	-	-	0.0	0.0	-	-
	Mansehra	10	40	+	+	0.0	0.0	-	-	0.0	0.0	-	-
Sindh	Karachi	06	24	+	+	02	08	+	+	0.0	0.0	-	-
	Hyderabad	03	12	-	-	03	12	-	-	0.0	0.0	-	-
	Sukkur	01	04	-	-	02	08	-	-	0.0	0.0	-	-
Punjab	Okara	02	08	+	+	01	04	+	+	04 (turkey and pigeon	16	+	+
	Faisalabad	10.0	0.3	+	+	07	28	+	+	02 (pigeon)	08	+	+
	Layyah	12	04	+	+	06	24	-	-	0.0	0.0	-	-
	Pakpattan	02	04	+	+	0.0	0.0	-	-	0.0	0.0	-	-
	Sheikhupura	09	36	+	+	02	08	-	-	0.0	0.0	-	-
	Sialkot	01	04	+	+	02	08	-	-	0.0	0.0	-	-
	Sahiwal	01	04	+	+	0.0	0.0	-	-	0.0	0.0	-	-
	Lahore	10.0	03	+	+	12	05	+	+	05 (peafowl and pigeon)	20	+	+
	Multan	03	12	+	+	0.0	0.0	-	-	0.0	0.0	-	-
	Jhang	02	08	-	-	02	08	-	-	0.0	0.0	-	-

¹birds kept under full human husbandry conditions and are fed with commercial food and supplements, ²Birds kept under partial human husbandry conditions and mainly reply on natural source of food. ³Wild birds that are kept under human control and provided commercial food and supplements. ⁴At least one of the studied flocks in particular district was positive, + and - indicate that samples were detected positive or negative by real-time PCR, respectively.

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ISSN: 1743-422X

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