Figure 3

RNA synthesis in SINV-infected cells after colchicine treatment. A. Localization of dsRNA complexes after colchicine treatment. SINV/NSP3-GFP-infected BHK cells (MOI = 1 pfu/cell), untreated or treated with colchicine from 1–6 hours post-infection were stained at 6 hours post-infection with mouse anti-dsRNA antibodies (Scientific Consultants) and visualized with donkey anti-mouse Alexa Fluor 595 secondary antibodies (red). Nuclei are stained with Hoechst 33342 (blue) and bars represent 10 μm. B. Northern blotting of lysates of SINV-infected BHK cells (MOI = 1 pfu/cell) after no treatment or treatment with 30 μM colchicine (from 1–24 hours post-infection) prepared at 24 hours post-infection. SINV-specific RNAs were detected by probing with a ³²P probe labeled by nick-translation of a plasmid containing the SINV structural protein genes.