Table 2 Primer sets used for RT-PCR amplification of the eight vRNAs of Ugandan influenza B isolates
From: Genetic analysis of influenza B viruses isolated in Uganda during the 2009–2010 seasons
Gene | Forward primer | Reverse primer | Source |
|---|---|---|---|
PB1 | Bm-PB1b-1: TATTCGTCTCAGGGAGCAGAAGCGGAGCCTTTAAGATG | Bm-PB1b-1200R: TATTCGTCTCGATGCCGTTCCTTCTTCATTGAAGAATGG | 1 |
PB1 | Bm-PB1b-1220: TATTCGTCTCGGCATCTTTGTCGCCTGGGATGATGATG | Bm-PB1b-2369R: ATATCGTCTCGTATTAGTAGAAACACGAGCCTT | 1 |
PB1 | BPB1-1103F: AAATACCTTGTCCTGATCTG | BPB1-1685R: TGGCATTTGTAGGTGTATCTAT | 2 |
PB2 | Bm-PB2b-1: TATTCGTCTCAGGGAGCAGAAGCGGAGCGTTTTCAAGATG | Bm-PB2b-1145R: TATTCGTCTCTCTCATTTTGCTCTTTTTTAATATTCCCC | 1 |
PB2 | Bm-PB2b-1142: TATTCGTCTCATGAGAATGGAAAAACTACTAATAAATTCAGC | Bm-PB2b-2396R: ATATCGTCTCGTATTAGTAGAAACACGAGCATT | 1 |
PB2 | BPB2-1045F: GGGAACGGAACAATACAGAAG | BPB2-1320R: CAAAAAATATCGTTGGAGTTG | 2 |
PA | Bm-PAb-1: TATTCGTCTCAGGGAGCAGAAGCGGTGCGTTTGA | Bm-PAb-1261R: TATTCGTCTCCCAGGGCCCTTTTACTTGTCAGAGTGC | 1 |
PA | Bm-PAb-1283: TATTCGTCTCTCCTGGATCTACCAGAAATAGGGCCAGAC | Bm-PAb-2308R: ATATCGTCTCGTATTAGTAGAAACACGTGCATT | 1 |
PA | BPA-510F GGGAGAGTGCTAAGCAGACT | BPA-1430R: TCCCATGCTGGCATTGCTTTCA | 2 |
PA | BPA-940F: GAAAAGTACTCAACACTAC | BPA-2020R TCCTTTAATGCTTGAATCAACAG | 2 |
HA | BHA-1F: ATGAAGGCAATAATTGTACT | BHA-700R: CATAATGTGTGGTCACTCC | 2 |
HA | BHA-673F: CCCCAGAAGTTCACCTCATC | BHA-1742R: CCCTTATAGACAGATGGAGC | 2 |
NP | MDV-B 5′ BsmBI-NP: TATTCGTCTCAGGGAGCAGAAGCACAGCATTTTCTTGTG | MDV-B 3′ BsmBI-NP: ATATCGTCTCGTATTAGTAGAAACAACAGCATTTTTTAC | 1 |
NA | Bm-NAb-1: TATTCGTCTCAGGGAGCAGAAGCAGAGCA | Bm-NAb-1557R: ATATCGTCTCGTATTAGTAGTAACAAGAGCATTTT | 1 |
NA | BNA-716F: GGGGAAATTGTTATCTTA | BNA-1410R: CCATTCCTCCATTACAGAGCC | 2 |
M | MDV-B 5′ BsmBI-M: TATTCGTCTCAGGGAGCAGAAGCACGCACTTTCTTAAAATG | MDV-B 3′ BsmBI-M: ATATCGTCTCGTATTAGTAGAAACAACGCACTTTTTCCAG | 1 |
NS | MDV-B 5′ BsmBI-NS: TATTCGTCTCAGGGAGCAGAAGCAGAGGATTTGTTTAGTC | MDV-B 3′ BsmBI-NS: ATATCGTCTCGTATTAGTAGTAACAAGAGGATTTTTAT | 1 |