Figure 3

IFN-γ production by CD3 + CD8+ cells in response to co-culture with HIV-infected human cells. IFN-γ responses of primary splenocyte cultures from HLA-A*0201 transgenic mice immunised with either HIV-v or NRP-v following co-culture with human syngeneic (T1) or allogeneic (H9) cells alone or infected with the HIV-1 IIIB strain were measured by flow cytometry. Panel A indicates the gating strategy applied to the analysis. Non viable cells and target cells were gated out prior measuring IFN-γ in CD3 + CD8+ cells. Panels B to D show the number of IFN-γ producing cells (events Y axis) vs the intensity of IFN-γ produced (X axis). The marker (M1) corresponds to the range of signal considered as a positive response. The response’s Geometric Mean (GM) and coefficient of variation (CV) as well as the % of events within M1 are also provided. Panel B corresponds to splenocytes (SPN) from NRP-v and HIV-v immunized groups untreated or treated with PMA + Ionomycin (PMA/I). Panel C corresponds to splenocytes co-cultured with allogeneic H9 alone or infected with IIIb HIV-1. Panel D corresponds to splenocytes co-cultured with syngeneic T1 cells alone or infected with IIIb HIV-1.