Immunopathological investigation and genetic evolution of Avian leukosis virus Subgroup-J associated with myelocytomatosis in broiler flocks in Egypt

Background Avian leukosis virus Subgroup-J (ALV-J) is a rapidly oncogenic evolving retrovirus infecting a variety of avian species; causing severe economic losses to the local poultry industry. Methods To investigate ALV-J, a total of 117 blood samples and 57 tissue specimens of different organs were collected for virological, and pathological identification, serological examinations, molecular characterization, and sequencing analysis. To the best of our knowledge, this is the first detailed report recorded in broiler flocks in Egypt. The present study targets the prevalence of a viral tumor disease circulating in broiler flocks in the El-Sharqia, El-Dakahliya, and Al-Qalyubiyya Egyptian governorates from 2021 to 2023 using different diagnostic techniques besides ALV-J gp85 genetic diversity determination. Result We first isolated ALV-J on chicken embryo rough cell culture; showing aggregation, rounding, and degeneration. Concerning egg inoculation, embryonic death, stunting, and curling were observed. Only 79 serum samples were positive for ALV-J (67.52%) based on the ELISA test. Histopathological investigation showed tumors consist of uniform masses, usually well-differentiated myelocytes, lymphoid cells, or both in the liver, spleen, and kidneys. Immunohistochemical examination showed that the myelocytomatosis-positive signals were in the spleen, liver, and kidney. The PCR assay of ALV-J gp85 confirmed 545 base pairs with only 43 positive samples (75.4%). Two positive samples were sequenced and submitted to the Genbank with accession numbers (OR509852–OR509853). Phylogenetic analysis based on the gp85 gene showed that the ALV-J Dakahlia-2 isolate is genetically related to ALV-EGY/YA 2021.3, ALV-EGY/YA 2021.4, ALV-EGY/YA 2021.14, and ALV-EGY/YA 2021.9 with amino acid identity percentage 96%, 97%; 96%, 96%; respectively. Furthermore, ALV-J Sharqia-1 isolate is highly genetically correlated to ALV-EGY/YA 2021.14, and ALV-EGY/YA 2021.9, ALV-J isolate QL1, ALV-J isolate QL4, ALV-J isolate QL3, ALV-EGY/YA 2021.4 with amino acid identity percentage 97%, 97%; 98%, 97%, 97%, 95%; respectively. Conclusions This study confirmed that ALV-J infection had still been prevalent in broilers in Egypt, and the genetic characteristics of the isolates are diverse. Supplementary Information The online version contains supplementary material available at 10.1186/s12985-024-02329-7.


Background
Avian leucosis virus (ALV) is an RNA virus belonging to the genus Alpharetrovirus, family Retroviridae, and induces a lot of neoplastic diseases with other reproduction troubles in different poultry species worldwide [1,2].Avian leucosis virus type J (ALV-J), strain HPRS-103, was first isolated from commercial meat-type chickens in the United Kingdom in the late 1980s [1,3].Moreover, in China, ALV-J infection was first detected in 1999 then followed by devastation to layers, of native breeds of chickens and ducks, causing catastrophic economic losses [4].To date, ALVs have been classified into 11 viral subgroups, based on their host range, as well as viral envelope interference and cross-neutralization patterns [5].Subgroups A-D, K, and J are exogenous viruses; that mainly infect chickens and turkeys, whereas subgroup E belongs to endogenous viruses.Subgroups ALV-A and ALV-B are common pathogens inducing lymphoid leukosis tumors with high incidence, while subgroups ALV-C and ALV-D have rarely affected the chicken [6].Recently, subgroup ALV-K induces fowl glioma [7].
Importantly, chickens infected with ALV-J usually exhibit tumor development, depressed immunity, growth retardation, reduced egg productivity, and a considerable increase in the morbidity and mortality rates that are most apparent in broiler breeder hens.ALV-J infection in breeder flocks is associated with the occurrence of myeloid leucosis (myelocytomatosis) [8].Myelocytomatosis was first observed in broiler breeder birds between 25 and 55 weeks.Furthermore, myelocytomatosis tumors are now being reported to appear in the field as early as 17 weeks [5,9].Numerous further cases of myelocytomatosis among broiler breeding flocks have also been reported in several European countries [10].America, Asia [4], Africa (Egypt) [11], and Australia [12].
In recent years, numerous strains of ALV Subgroup-J have been isolated from white meat-type chickens; causing a serious impact on the growth performance of the poultry industry [3].Due to horizontal and vertical transmission, ALV-J has caused increasingly severe damage to the poultry industry worldwide as infected broilers exhibit various tumor phenotypes, such as hemangioma, myeloid leukosis, and myelocytoma with decreased weight gain [13,14].Moreover, in other parts of the world as China, the mixed structure of the chicken breeding industry through crossbreeding Chinese local breeds with other western breeds may potentially be of concern in increasing the high frequency of ALV-J infection, especially in the case of vertical transmission to their progeny [15].In Egypt, ALV-J-induced myelocytomatosis was reported in broilers − 28 days old-depending on histopathological lesions and antibody detection as the positive samples were (26%) with a mortality rate was (3.5%) [11,16].Taken into account, myelocytomatosis diagnosis is based on gross pathological lesions and antibody titer in a 27-week-old broiler breeder.Additionally, in laying hens, ALV-J particles are presented abundantly in the ovarian stroma, bud from cells in direct contact with oogonia, and oocytes with the highest concentration in the albumen-secreting glands of the magnum.This early ovarian and oviduct exposure may cause an early and diffuse infection [17].Moreover, monoclonal antibodies against ALV-J envelop glycoproteins have been produced with broad reactivity for most ALV-J isolates.These antibodies have been used to determine tissue tropism of ALV-J naturally infected chickens [18].
Currently, there is no vaccination strategy or specific treatment available for ALV-J.Thus, control measures depended on the elimination of positive cases, management approaches and biosecurity programs in the poultry farms to decrease ALV spreading and clinical symptoms [19].Therefore, rapid and confirmatory diagnosis is necessary to eradicate ALV from breeding flocks [20].The ELISA is a useful serological diagnosis for the detection of ALV-J antibodies as it is a sensitive, easy, safe, and rapid diagnostic tool [11].ELISA diagnostic method of ALV-J is reported to have sensitivity (99.2%), and specificity (100%), and can be used clinically for screening purposes [21].
Recently, molecular-based diagnostic techniques including insitu hybridization, PCR, and sequencing analysis have been developed for ALV detection [22].Particularly, the proviral DNA arrangement of the ALV genome involves three important structural proteins (gag, pol, and env) which have been translated into the specific group antigen and envelop glycoproteins.The gp85 envelop glycoprotein, is closely associated with the viral entry, and host range, inducing host-neutralizing antibodies, tissue tropism, and virulence.Moreover, it is the major subgrouping determinant responsible for host infection and tumor formation [23][24][25].In addition, the gp85 gene is the most variable region of the envelope which evolves more rapidly in ALV-J compared to the other subgroups causing serious economic losses.Thus, it is crucial to monitor the gp85 gene evolution continuously to update any new strains and mutations [19,26,27].
Taken together, the present study targets the molecular characterization, serological assay, and sequencing analysis of ALV-J isolates that are circulating in broiler flocks in El-Sharqia, El-Dakahliya, and Al-Qalyubiyya Egyptian governorates through PCR technique, ELISA, and molecular sequencing approaches.Our study also involved the myelocytomatosis diagnosis in naturally infected broiler chickens with a complete pathological and immunohistochemical picture of different infected organs.

History of examined farms and sampling
A total number of 18 broiler farms of different breeds, aged from 5 to 7 weeks old, were investigated during the period from November 2021 to April 2023.Capacity of farms ranged from 5000 to 15,000 birds/farm.The broiler farms were located in the Lower Egypt region in 3 Governorates; El-Sharqia, El-Dakahliya, and Al-Qalyubiyya (Table 1).Collectively, a total of 117 blood samples were collected from suspected diseased birds for serological examination using the ELISA test.Besides, all positive samples for the ELISA test are recommended for PCR tests and histopathological examination, thus we selected a total of 57 tissue specimens of positive cases including liver, spleen, and kidney.The sampled flocks were recently diseased birds that had shown depression, anorexia, and growth retardation.

Virus isolation assays
The chicken embryo rough (CER) cell line was purchased from VACSERA (Vaccine and Serum Association), Dokki, Giza, Egypt for virus isolation and culturing.Samples were processed and then inoculated in CER cells with continuous monitoring for the evidence of virus growth.The inoculated cells were incubated at 37 °C with 5% CO 2 for 5 days for each passage.An uninfected CER cell line is considered a negative control.Cell cultures are noticed daily for any cytopathic effects (CPE) according to the method described by [16,28].After three serial cell passages, the culture supernatants containing the virus were harvested to confirm by PCR test [25].Concerning embryonated chicken eggs (ECE) inoculation, the collected tissue specimens (liver, spleen, and kidney) were processed for virus isolation.The processed samples were inoculated in 9-day-old specific-pathogen-free (SPF)-ECE, then incubated at 37 °C for 5-7 days, after that examined daily by candling with recording any mortality cases.The embryos were opened aseptically for gross lesion examination [29].Consequently, the amniotic fluid was harvested and then tested using specific PCR analysis.

Serological assay
The collected blood samples, from the wing vein, were centrifuged at 3000 rpm for 15 min for serum separation.Sera were stored at − 20 °C until used for detection of the anti-p27 antibody developed against ALV-J in the serum of all diseased chickens using an antigen capture ELISA (ELISA Test Kit, IDEXX Laboratories, Inc., USA).The results interpretation was performed according to [16,29].In addition, the difference of optical density (OD) between antigen-coated wells and serum sample, in which the sample to the positive ratio (S/P) ratio was expressed as follows: S/P ratio = (OD of sample − OD of negative control)/(OD of positive control − OD of negative control).Samples with an S/P ratio of 0.6 or greater were considered positive.

Proviral DNA extraction and PCR analysis
Tissue samples (liver, spleen, and kidney) were kept at − 70 °C until used for DNA detection of ALV-J by PCR amplification using Phusion ® High-Fidelity DNA Polymerase (Thermo, MA, USA) regarding the manufacturer's methods.The DNA extraction was carried out according to [30].The oligonucleotide primers used in PCR amplification of ALV-J included; the forward primer H5 was annealed from the 3′ region of the pol gene which was conserved mainly across ALV subgroups.The reverse primer H7 was annealed specifically from a wellconserved region of the gp85 of ALV-J.This pair of primers for ALV-J gives a PCR amplification product size in 545 base pairs (bp) [31].The primers H5 and H7 originated from the HPRS-103 ALV-J prototype strain (Genbank accession No. Z46390) (Table 2).

DNA sequencing and phylogenetic analysis
The two positive amplicons were completely purified using the QIAquick Gel Purification Kit (Qiagen, Hilden, Germany), then sequenced using the BigDye Terminator v3.1 Cycle sequencing Kit (Applied Biosystems, California, USA) via specific primers for gp85 of ALV-J.The oligonucleotide sequence was established using ABI 3500 Genetic Analyzer (Life Technologies, California, USA).The nucleotide and amino acid   Our isolates from this study are 1-2.Isolates 3-4 are British and American ALV-J reference strains.Isolates 5-17 are American ALV-J reference strains.sequences were aligned with other related strains in Genbank using the Clustal W program.A phylogenetic tree was designed using the MEGA-X program [32] and BioEdit software packages, with levels assessed using 1000 bootstrap replicates [33].The sequences of the ALV-J strains in Genbank isolated from broilers, layers, and breeders were comprised in the multiple-sequence alignment and then summarized in (Table 3).

Analysis of the recombination events
Recombination events of each ALV-J strain isolated in our study were investigated according to [34] (RDP-5 Program), the used algorisms including BootScan, MaxChi, GENECONV, SiScan, Chimaera, LARD, RDP5, Phyl-Pro, and 3Seq, were utilized for comparison [35].Recombination events were maintained by four independent approaches or more; which were only considered as accurate positive events.

Histopathological examination
Tissue specimens (lung, liver, spleen, heart, kidney, proventriculus, gizzard, and bone) were collected for histopathological examination.These tissue specimens were fixed with (10% neutral buffered formalin), washed, dehydrated, and embedded in paraffin.Paraffin blocks were sectioned at 5 µm thickness and stained with hematoxylin and eosin (H&E), and also Giemsa stain was used when needed [36].

Immunohistochemical examination
To detect the presence of ALV-J antigen, tissues were fixed with 10% buffered neutral formalin, paraffinembedded, sectioned with 4 microns' thickness, and mounted on poly-l-lysine-coated slides.The tissue sections were stained with a routine streptavidin-biotin/ horseradish peroxidase (HRP)-conjugated immunohistochemical technique.The sections were prepared and stained to be examined microscopically with light microscopy according to the method described by [37].

Clinical findings and mortality incidence
Clinical signs in 18 broiler farms of different breeds, aged from 5 to 7 weeks old, in the Lower Egypt region were mostly non-specific.The sampled broiler flocks had shown depression, anorexia, growth retardation, weakness, and dehydration.The mortality rate was recorded at about 7% while the morbidity was about 20%.

Virus isolation on tissue culture and SPF-ECE
The characteristic CPE does not appear up to the second passage level.The prominent CPE of inoculated ALV-J appears at the 3rd passage.Also, ALV-J CPE was observed after 72 h (h) in inoculated CER cell culture.CER cell culture showed aggregation which progressed rapidly; also rounding and degeneration (Fig. 1B).On the 5th day post-inoculation (d.p.i), there were enormous detachments of cells.Whereas, the uninfected control cells showed no changes (Fig. 1A).On the other hand, ALV-J was inoculated successfully in the SPF-ECE.After 7 days, mortality and gross pathological lesions of the inoculated embryos were observed; including embryonic death after 48 h p.i. and also the survived embryo on 7th d.p.i showed stunting, curling, dwarfing (Fig. 2B), anomalies, hemorrhages on the body surface, enlarged liver (Fig. 2A), and congestion of the chorioallantoic membrane (CAM).Antigen capture ELISA The ELISA results are calculated based on the S/P ratio.Serum samples giving an S/P ratio of 0.6 or greater were considered positive.Collectively, serum samples of broiler farms at different ages revealed that among 117 serum samples, only 79 samples were positive for ALV-J (67.52%), with a mean of 0.95 for the S/P ratio of sera collected from diseased flocks.Meanwhile, 38 samples were negative for ALV-J (32.47%) with a mean of 0.38 for the S/P ratio of sera collected from healthy flocks (Additional file 1).

Molecular identification
Collectively, 57 tissue samples (liver, spleen, and kidney) are examined from 14 farms with positive ELISA for ALV-J.PCR results showed that only 43 samples were positive (75.4%) (Fig. 3).These PCR results for different organs are summarized in (Table 4).The culture supernatants and amniotic fluid samples were tested by PCR as shown in (Fig. 4).

Avian leukosis virus strain GDFX0601
ALV-K  Fig. 5 Collective phylogenetic tree based on gp85 gene sequences alignment of ALV-J with other reference sequences.The phylogenetic analysis of the ALV-J gp85 gene revealed that our ALV-J two isolates located in subgroups II (ALV-J Dakahlia-2 and ALV-J Sharquia-1) with other Egyptian strains cluster in the same group (Subgroups II).The ALV-J two isolates in our study are indicated by a triangle.The tree was constructed by the neighbor-joining method with 1000 bootstrap replicates, using MEGA 7.0 of 73%-75%, and 75%-77%; respectively (Table 5).Our Egyptian ALV-J isolates (ALV-J Dakahlia-2 and ALV-J Sharqia-1) were submitted to Genbank with accession numbers (OR509852-OR509853).Amino acid sequencing analysis of the gp85 gene of our two isolates revealed 96% similarity to each other, also 96% similarity based
ALV strain ALV-J isolate QL6 gp85 protein gene ALV strain ALV-J isolate QL2 gp85 protein gene ALV strain ALV-J isolate QL3 gp85 protein gene ALV strain ALV-J isolate QL1 gp85 protein gene ALV isolate ALV-J -Dakahlia-2 ALV strain ALV-J isolate QL5 gp85 protein gene ALV strain ALV-J isolate QL4 gp85 protein gene ALV isolate ALV-J -Sharqia-1   Bold values refer to our ALV-J Egyptian samples Nucleotide identities and divergence of sequenced virus isolates comparable to other selected strains from China, Russia, Egypt, the United Kingdom, and the USA.The figure reveals a comparative alignment of the gp85 gene in which, the gp85 nucleotide identity percentage of two of our Egyptian isolates ranges from 71 to 100% comparable to other different reference strains.Besides, the amino acids identity percentage of two of our Egyptian isolates ranges from 77 to 98% comparable to other different reference strains on nucleotide identity level.In particular, no evidence of variations or amino acid mutations of our isolates in the gp85 gene were detected in the putative variable regions, vr2 (Fig. 7).Regarding prototype strain HPRS103 (UK) and ADOL-7501 (USA) reference strains comparison, the sequencing analysis in our study clustered our two ALV-J isolates into subgroups II with other strains, as shown in (Fig. 6).

Analysis of the recombination events
According to the RDP5 Program, the recombination events in the gp85 gene sequence of the two ALV-J Egyptian isolates were analyzed.There is no evidence of recombination detected in two ALV-J isolates (ALV-J Sharquia-1 and ALV-J Dakahlia-2).

Pathological findings Macroscopical findings
Necropsy mostly was not clear except in some cases showed off-white masses on the inner surface of both the sternum and pelvis (Fig. 8a, b).The tumors occur as soft and friable.

Microscopic findings
Liver Tumors consist of masses of uniform, usually well-differentiated, myelocytes.Their nuclei appear as large, distinct, vesicular, and eccentrically located.The cytoplasm is saturated with the acidophilic spherical granules.The hepatic lobules showed multifocal aggregations of mature granulated myeloid cells with mitotic figures.The hepatocytes appeared as solitary islets inside the massive infiltration of myeloblastic cells.In some cases, myeloid and lymphoid tumors were detected in the same hepatic tissue (Fig. 9A).The lymphoblastic cells are large mononuclear cells homogenous in size with poorly defined cytoplasmic membrane and basophilic cytoplasm.The nuclei appeared vesicular in which margination and clumping of chromatin with the appearance of one or more obvious acidophilic nucleoli.These neoplastic cells showed mitotic figures (Fig. 9B).Kidneys The renal tubules were separated from each other by massive infiltration of lymphoblastic cells.Renal tubules adhering to neoplastic cell masses suffer from degenerative changes and pressure atrophy.Diffuse granulated myeloid cells were seen to infiltrate the renal parenchyma causing pressure atrophy and loss of renal tubules (Fig. 9C).Nephroblastoma is capsulated by a dense fibrous capsule, the stromal tissue shows ill distinct glomeruli.Tumor tissue showed undifferentiated cystic renal tubules.The tubules are lined by multilayer epithelial cells.Most of the tubules contain hemorrhagic blood.The epithelial cells penetrating the connective tissue storms forming newly formed tubules (Fig. 9D).
Spleen Proliferation of lymphocytes that show pleomorphism, atypism, and mitotic activity.Diffuse proliferation of large lymphoid cells allover splenic splenic tissue especially red pulp was seen in some cases (Fig. 9E).Few myeloid cell infiltrations together with lymphoid cells were recorded (Fig. 9F).Proliferation of Bone and myelocytoma Proliferation of granulated myelocytes in the bone marrow of various bones and in the periosteum of the ribs, pelvis, and skull (Fig. 9G, H) and sternum (Fig. 9J).Proliferation begins in the bone marrow of epiphysis.The myelocytes invaded from the bone marrow to periosteal areas through Haversian and Volkmann's canals.Additionally, there is a wall thickening and lumen narrowing of the sternal bone together with dentation in the periosteum which is considered osteopetrosis.The sections taken from the tumorous growths over the ribs, sternum, and pelvic bones revealed a marked proliferation of solid masses of uniform mature myelocytes (Fig. 9I).

Immunohistochemical findings
Immunohistochemistry was performed using the anti-ALV-J specific antibody to detect the myelocytomatosis positive signals (antigen) in different organs.The myelocytomatosis positive signals were indicated by the brown staining.The results showed that the ALV-J positive signals were mainly presented in the spleen, liver, and kidney.In the spleen, staining was greatest in the splenic trabeculae, subcapsular sinuses, and lymphoid follicles (Fig. 10A-D).In the liver, staining was intense in hepatocytes around congested blood vessels, infiltrated lymphoid cells, and subcapsular sinuses (Fig. 10E-H).In the kidney, a strong positive reaction in the lining epithelium of renal tubules, glomerular cells, and under the renal capsule was detected (Fig. 10I, J).In other tissues, there is no staining (Additional file 2).

Discussion
ALV-J was a great threat causing huge economic losses in the poultry industry worldwide.Notably, ALV-J spread rapidly through poultry populations, and the emergence viral new strains has been spread rapidly among different countries thus, viral eradication is very necessary for commercial breeding flocks.ALV-J Infection is challenging to control without a vaccination program, thus the only obtainable control method is flock condemnation; certainly, China has managed and controlled ALV-J infection by cautiously selecting non-infected breeders for broiler and layer industry [25,38,39].Interestingly, in Egypt, ALV-J spreads quickly during 2014 throughout Egyptian poultry flocks including native and foreign breeds of layers and ducks, with high mortality rates [16,40].Recently, sporadic cases of ALV-J were detected in Egypt in poultry and ducks with various genetic backgrounds [19,41].
In this study, broiler flocks in highly poultry production three governorates (El-Sharqia, El-Dakahliya, and Al-Qalyubiyya) have shown general clinical symptoms with suspicion of viral natural infections.Also, the mortality rate was recorded at about 7% while the morbidity was about 20%; with non-specific necropsy except for offwhite masses on the sternum and pelvis in some investigated birds.To the best of our knowledge, no research or report has been achieved mainly on ALV-J associated with myelocytomatosis in broiler flocks, in Egypt.
According to our findings, ALV-J CPE was observed after 72 h in inoculated CER cell culture as aggregation, rounding, ballooning, degeneration, and enormous detachments of cells.These results were in line with the CPE findings of [16,28].Also, [42] mentioned that cytopathic ALV strains inoculated in chicken embryo fibroblast cells have given CPE and cell detachment 3 days after infection.Concerning egg inoculation, ALV-J pathological lesions on SPF-ECE were mainly stunting, curling, anomalies, and enlarged liver.These lesions may be due to the ALV-J direct effect.Embryo mortality increased as the virus passing increased until the third passage.ALV-J has been reported to cause severe hemorrhage and embryo death within 4-5 days following embryo infection.These results came in accordance with [29] At the same time, results analysis obtained from the serological survey showed that 14 farms were positive for ALV-J (77.7%).The virus identification using antigen capture ELISA revealed that only 79 serum samples were positive for ALV-J (67.52%) based on the S/P ratio.These subsequent results are in agreement with [43,44].In addition, these findings are nearly in agreement with [16] who reported that the positive ELISA results of collected serum samples reach to 74.2% in broiler chickens.Moreover, [11] stated that ALV-J antibody titer was significantly (P < 0.01) increased in experimentally infected SPF chicks of one-day-old from the 3rd-month post-infection (mpi) till the 5th mpi (experiment end).Interestingly, our promising results confirm the high incidence of ALV-J in the currently examined farms indicating a vertical transmission of the causative virus.We first followed up on the ALV-J infected breeder hens and then confirmed by histopathology examination (unpublished data), after that all clinical specimens were collected from affected broiler flocks.However, [45] suggested that the hatching of one-day-old egg-type chicks with ALV-J-infected meat-type chicks in the same hatchery had contributed to horizontal infection.
Despite evidence of virus growth in SPF-ECE embryo and tissue culture, PCR is the most appropriate and rapid method to detect ALV-J; providing epidemiological data of various isolates prevalent in infected flocks periodically.Analysis of PCR assay with ALV-J specific primers from (the liver, spleen, and kidney) revealed that only 43 samples were positive with a percentage of 75%.Currently, the liver and spleen show very high tropism for ALV-J according to PCR results at 91.1% and 89.4%; respectively.These results are similar to those [46] who recorded the ALV-J high tropism in the different visceral organs, especially the liver and spleen.This result indicated that these samples were collected from viremic-tolerant chickens.Parallel to our results, [11,16] reported that all examined samples obtained from the liver, spleen, and kidney indicated a positive reaction with ALV-J at 545 bp.Also, [19] mentioned that ALV-J infection was detected in the Lower Egypt layer farms in El-Qalyubia, El-Monofia, El-Gharbia, El-Behera, and El-Daqhlia governorates.Regarding our results, [41] reported also different rates of ALV-J infections in the different governorates (Gharbia, Damietta, Sharkia, and Dakahlia) based on qRT-PCR in collected breeder chickens and duck samples.Our results indicated that ALV-J is the main etiology of viral tumors in broilers at these three El-Sharqia, El-Dakahliya, and Al-Qalyubiya governorates.Regarding previous studies, the gp85 encoding protein is highly evolved and capable of receptor-binding site, which plays a critical role in viral entry that determines the host ranges and tumor types [24,25].To investigate the genetic evolution of the gp85 gene in our ALV-J isolates, they were sequenced, systematically analyzed then compared to other ALV-J reference sequences.The genetic characteristics of the reported Egyptian strains (ALV-J Dakahlia-2 and ALV-J Sharqia-1) were highly similar to other ALVs.Phylogenetic analysis indicated that the ALV-J Dakahlia-2 isolate has the highest genetically related to Egyptian isolates as ALV-EGY/YA 2021.3,ALV-EGY/YA 2021.4,ALV-EGY/YA 2021.14, and ALV-EGY/YA 2021.9 with nucleotide identity percentage 100%, 97%, 96%, 96%; respectively, and on the amino acid level were with 96%, 97%; 96%, 96%; respectively.Furthermore, ALV-J Sharqia-1 isolate is highly similar to other Egyptian strains like ALV-EGY/YA 2021.14, ALV-EGY/ YA 2021.9,ALV-J isolate QL1, ALV-J isolate QL4, ALV-J isolate QL3, ALV-EGY/YA 2021.4 with nucleotide identity percentage 98%, 98%, 98%, 98%, 97%; respectively, and on the amino acid level were with 97%, 97%; 98%, 97%,97%, 95%; respectively.In particular, our previous results may suggest that these prevalent ALV-J isolates might be of the same sources or have similar ancestors.This reasonably elevated detection rate might be attributed to the vertical transmission of the.ALV-J infection.Meanwhile, ALV-J Dakahlia-2 and ALV-J Sharqia-1 isolates shared 73-75% homology with the American strain (ALJ-10022-2).Our findings are not in agreement with [47] who reported that the gp85 of PK19SA01 shares 95.5% identity with the American strain.Also, ALV-J Dakahlia-2 and ALV-J Sharqia-1 isolates shared 91% -93% similarity with the American reference strain (ALJ-ADOL-7501).These subsequent results are not in agreement with [19] who mentioned that the Egyptian ALV-J isolates were similar to HPRS-1003 (prototype strain) with an identity percentage of 91.2-91.8%.Importantly, we can speculate that our Egyptian ALV-J strains might be introduced from America through chicken breeding.On the other hand, ALV-J isolates were distinctly apparent from Chinse isolates with 71% and 73% identity.These results are not parallel to [19] who stated that the nucleotide identity percentage of their Egyptian isolates was within the range of 88-94% when compared to Chinese reference strains.
The core region of the gp85 gene contains five variable regions (hr1, hr2, vr1, vr2, and vr3) [48].These regions (hr1, hr2, and vr3) are ALV-J receptor interaction determinants [49].Previous studies revealed that the gp85 gene tends to mutate as a result of immune pressure, causing a lot of changes in antigenic properties and virulence [20].In the present study, no evidence of variations or amino acid mutations of our isolates in the gp85 gene were detected in the putative variable regions, vr2.These current findings are similar to those [19] who recorded that no changes existed in the vr2 domain of the gp85 gene of their isolates at the layer farms.In contrast, these results are not in agreement with [41] who reported 25 true SNPs among the five strains of chicken and duck breeders, in which only 5 SNPs lead to amino acid mutation.These amino acid substitutions might cause variations in the pathogenicity, oncogenicity, and ALV-J host range.Finally, our findings act as a warning that the ALV-J eradication is not disposable, so continuous monitoring is essential.
The histopathological picture besides the molecular characterization gave an accurate diagnosis for ALV-J.ALV-J could induce malignant or benign tumorigenic diseases and immunosuppressive responses in poultry such as hemangiomas, myelomas, and myelocytomatosis.Tumor development is a multi-step process representing the abnormal expression of an apoptotic gene, inactivation of tumor suppressor genes, or activation of proto-oncogenes [50].Myelocytomatosis causes high economic losses in white meat-type breeder farms [51].Furthermore, myelocytomatosis is a tumor disease in which tumor progress is a process that is complicated and related to many factors as genetic background, immune competence, and viral infection factors.The process of promoting carcinogenesis is still unknown, but the integration of the myelocytomatosis provirus may interfere with the function of the host endogenous gene [52,53].
The immune suppression due to myelocytomatosis may involve atrophy of lymphoid organs, decreased mitogeninduced blastogenesis, and decreased antibody response [54].Interestingly, the immune system alteration occurs as a result of cessation of B cell maturation in addition to a blockage in the development of T-suppressor cells, probably due to hindrance with functional IL-2 synthesis [55,56] showed that ALV-J could induce lymphocyte apoptosis in immune organs, particularly in young chickens.Lymphocyte death increases susceptibility to other diseases.
Myelocytomas have a characteristic gross appearance in which myelocytes proliferate and soon overgrow the bone marrow.Tumors are formed by the expansion of marrow growth and may crowd through the bone and periosteum.They occur frequently on the surface of bone such as the costochondral junctions of the ribs and on the sternum and pelvis, these aforementioned lesions agree with [46,57].
Histopathological evaluation of the liver concluded the presence of myeloid and lymphoid.The features of the liver lesions were aggregations of mature granulated myeloid cells.The neoplastic cells replaced the hepatocytes with relative atrophy of the surrounding cells.The same findings were recorded by [11,37].Meanwhile, focal aggregations of lymphoblastic cells were detected in the hepatocyte.Furthermore, In the liver, the brown granules resembled a positive reaction for specific viral particles present in the Kupffer cells and lymphocytes as well as erythroblasts; this supports the previous findings which were also confirmed by detecting the virus from liver tissues using PCR.These results agree with [5,41,53,58,59].
In contrast, the microscopic picture of spleen sections showed that ererea a pleomorphic lymphoid population surrounding arteriole.By Giemsa stain, myeloid cells appear obviously with their eosinophilic granules.Viral antigen was greatest in the splenic trabeculae, subcapsular sinuses, and lymphoid follicles by IHC.These results were supported by [37] who recorded ALV-J positive signals in the erythroblast cytoplasm, spleen, lung, and other tissues especially rich in blood.
The microscopic examination of the kidney revealed multifocal neoplastic aggregations of granular myeloid cells.The tumor cells were aggregated between the degenerated renal tubules and around the congested blood vessels.Granulated myeloid cells were seen infiltrating the interstitial tissue causing pressure atrophy and loss of some renal tubules when stained with Giemsa stain, that result was confirmed by [41,58,60,61].
The proliferation of granulated myelocytes was detected in the bone marrow and the periosteum of the sternum.Proliferation begins in the bone marrow of epiphysis.The Myelocytes invaded from the bone marrow to periosteal areas through Haversian and Volkmann's canals.Myelocyte proliferation was also detected in the bone marrow.Additionally, there is a wall thickening and lumen narrowing of the sternal bone together with dentation in the periosteum which is considered as osteopetrosis.Finally, ALV-J tropism for chicken bone marrow cells, and induces their neoplastic transformation [62].This finding agrees with that recorded by [11,63,64].

Conclusions
We first isolated two ALV-J strains associated with myelocytomatosis from broiler flocks, in Egypt.In summary, the circulating ALV-J infection associated with myelocytomatosis during 2023 in broiler flocks at different localities of Egypt was diagnosed through PCR technique, serological assay, molecular sequencing approaches, pathological, and immunohistochemical examinations.ALV-J causes neoplastic diseases in broiler flocks, with the highest rate of infection presented in these governorates as El-Sharqia, El-Dakahliya, and Al-Qalyubiyya.In addition, the ALV-J gp85 gene evolution of our isolate (Dakahlia-2, identified as subgroup II) have the highest genetically related to ALV-EGY/YA 2021.3,ALV-EGY/YA 2021.4,and ALV-EGY/YA 2021.14 with nucleotide identity percentage 100%, 97%, 96%; respectively, and on the amino acid level were with 96%, 97%; 96%; respectively.Moreover, ALV-J Sharqia-1 isolate is highly similar to ALV-EGY/ YA 2021.14, ALV-EGY/YA 2021.9, and ALV-J isolate QL1 with nucleotide identity percentage of 98%, and on the amino acid level were with 97%, 97%; 98%; respectively.Our Egyptian ALV-J isolates (ALV-J Dakahlia-2 and ALV-J Sharqia-1) were submitted to Genbank with accession numbers (OR509852-OR509853).The phylogenetic analysis based on the nucleotide and deduced amino acid sequences of the gp85 gene showed no evidence of variations or amino acid mutations in the putative variable domains, vr2.Currently, no vaccinations or treatments for ALV are presented and such ALV still threatens the local poultry industry.This reminds us to eradicate the positive cases, strengthen the breeder introduction detection, and apply periodic molecular monitoring for all recent Egyptian strains.Furthermore, the whole genome sequencing of these isolates is recommended to detect both the pathogenicity and antigenicity of these circulating ALV-J strains.

Fig. 1
Fig. 1 ALV-J isolation on CER cells.A The normal spindle uninoculated CER cells.B CER cells showaggregation, degeneration, and detachment of cells (Magnificent power is 10×)

Fig. 2 Fig. 3
Fig. 2 Macroscopic lesions from internal organs of an embryo with ALV-J associated with myelocytomatosis; A Chick an embryo showing an enlarged liver.B Chick embryo showing stunting and curling

Fig. 6
Fig.6 Detailed phylogenetic tree based on gp85 gene sequences alignment of ALV-J.This phylogenetic analysis of the ALV-J gp85 gene confirms that our ALV-J two isolates located in subgroup II are comparable to other strains deposited in Genbank

Fig. 7
Fig. 7 The gp85 sequence comparisons for the two ALV-J Egyptian isolates and reference strains.The top line represents the amino acid numbers in the gp85 sequence of HPRS-103.The letters indicate amino acid substitutions.The dots (.) indicate identical amino acids, dashes (−) indicate gaps produced in the alignment, and putative variable regions (Vr2) are indicated in red boxes and marked

Fig. 8 Fig. 9
Fig. 8 Macroscopic lesions from internal organs of broiler chickens naturally infected with myelocytomatosis; A (left): Costo-chondral junction of 7 weeks old broiler chicken showing whitish myelocytomas (arrow), and B (right): Pelvic bone of 7 weeks old broiler chicken showing whitish myelocytomas on the inner surface (arrow)

Fig. 10
Fig. 10 Immunoreactivity photomicrograph sections from the spleen, liver and kidney of broiler chickens naturally infected with myelocytomatosis; A Spleen showing staining of sub-capsular area that extending to the splenic trabeculae (arrow) and around lymphoid follicles (Scale bar: 100 µm), B Spleen showing staining of lymphoid follicle (Scale bar: 20 µm), C Spleen showing staining of connective tissue capsule (arrow) (Scale bar: 50 µm), and D Spleen showing staining of lymphoid tumor cells scattered within white pulp (Scale bar: 50 µm).E Liver showing staining of the hepatic parenchyma, especially around congested sinusoids (Scale bar: 50 µm), F Higher magnification of the previous photo showing antigen staining (arrow) (Scale bar: 20 µm), G Liver showing staining of the liver capsule and subcapsular area (Scale bar: 50 µm), H Liver showing staining around the central vein (Scale bar: 50 µm), I Kidney showing staining of the lining epithelium of renal tubules (arrow).(Scale bar: 50 µm), and J Kidney showing staining of inter-tubular area (Scale bar: 20 µm)

Table 1
The descriptive flock data of the investigated broilers for ALV-J detection from 2021 to 2023 in the El-Sharqia, El-Dakahliya, and Al-Qalyubiyya

Table 2
Sequence of oligonucleotide primers, targets, and expected PCR product sizes

Table 3
ALV-J referential strains used in this study

Table 4
Detection of ALV-J proviral DNA in different organs of broiler flocks using PCR

Table 5
Nucleotide identities and divergence of sequenced virus isolates comparable to other selected strains