Table 1 PCR primers for HA and NA full genome sequencing of A(H1N1) pdm09 and A(H3N2)
Primer name | Primer sequence (5’–3’) |
|---|---|
A(H1N1) pdm09 | |
pdmHA-I-F4 | TGTAAAACGACGGCCAGTAAAAGCAGGGGAAAACAAAAGC |
pdmHA-I-R1049 | CAGGAAACAGCTATGACCYGGGRCATTCCTCAATCCT |
pdmHA-II-F936 | TGTAAAACGACGGCCAGTGCTATAAACACCAGCCTCCC |
pdmHA-II-R1756 | CAGGAAACAGCTATGACCCTCATGMTTCTGAAATCCT |
pdmNA-I-F9 | TGTAAAACGACGGCCAGTCAGGAGTTTAAAATG |
pdmNA-I-R869 | CAGGAAACAGCTATGACCAGGRTARCAGGAGCATTCCTC |
pdmNA-II-F741 | TGTAAAACGACGGCCAGTRTAATGACYGAYGG |
pdmNA-II-R1457 | CAGGAAACAGCTATGACCAGTAGAAACAAGGAGTTTTT |
A(H3N2) | |
H3-I-F2 | TGTAAAACGACGGCCAGTGCAAAAGCAGGGGATAATTC |
H3-I-R964 | CAGGAAACAGCTATGACCTTTTGRAATGGTTTGTCATTGG |
H3-II-F868 | TGTAAAACGACGGCCAGTAAGCTCRATAATGAGRTCAGAT |
H3-II-R1788 | CAGGAAACAGCTATGACCAGTAGAAACAAGGGTGTTTT |
N2-I-F1 | TGTAAAACGACGGCCAGTAGCARAAGCAGGAGT |
N2-I-R982 | CAGGAAACAGCTATGACCAAGTCCTGAACACACATAAC |
N2-II-F879 | TGTAAAACGACGGCCAGTTCAGATGTRTHTGCMGAGAC |
N2-II-R1465 | CAGGAAACAGCTATGACCAGTAGAAACAAGGAGTTTTT |