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Fig. 1 | Virology Journal

Fig. 1

From: Pathogenicity and immunogenicity of gI/gE/TK-gene-deleted Felid herpesvirus 1 variants in cats

Fig. 1

Construction of recombinant FHV WX19 with deletion of the gI, gE, and TK genes. A Diagram of the FHV TK, gE, and gI gene deletions. The transfer plasmids pT–eGFP and pT–mCherry were constructed for homologous recombination with the cotransfection of sgRNAs, followed by the infection of cells with FHV strain WX19. B FHV ∆gIgE eGFP, FHV ∆TK mCherry and FHV ∆gIgE/TK eGFP–mCherry show light field and and florescence dark field, respectively. C CRFK cells were infected (MOI = 0.1) with FHV WX19 (line 5), FHV ∆gIgE eGFP (line 2), FHV ∆TK mCherry (line 3), FHV ∆gIgE/TK eGFP–mCherry (lane 4), or mock infected (lane 1). At 48 h postinfection (hpi), the levels of the gI, gE, TK, and gB proteins were determined with western blotting. D One-step growth curves of the recombinant viruses in CRFK cells

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